2w5e

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{{Seed}}
 
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[[Image:2w5e.jpg|left|200px]]
 
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==Structural and biochemical analysis of human pathogenic astrovirus serine protease at 2.0 Angstrom resolution==
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The line below this paragraph, containing "STRUCTURE_2w5e", creates the "Structure Box" on the page.
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<StructureSection load='2w5e' size='340' side='right'caption='[[2w5e]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w5e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_astrovirus_1 Human astrovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W5E FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_2w5e| PDB=2w5e | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5e OCA], [https://pdbe.org/2w5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w5e RCSB], [https://www.ebi.ac.uk/pdbsum/2w5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w5e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q3ZN04_HASV1 Q3ZN04_HASV1] Protein covalently attached to the 5' extremity of the genomic and subgenomic RNAs.[ARBA:ARBA00029406]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w5e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w5e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Astroviruses are single-stranded RNA viruses with a replication strategy based on the proteolytic processing of a polyprotein precursor and subsequent release of the viral enzymes of replication. So far, the catalytic properties of the astrovirus protease as well as its structure have remained uncharacterized. In this study, the three-dimensional crystal structure of the predicted protease of human pathogenic astrovirus has been solved to 2.0 A resolution. The protein displays the typical properties of trypsin-like enzymes but also several characteristic features: (i) a catalytic Asp-His-Ser triad in which the aspartate side chain is oriented away from the histidine, being replaced by a water molecule; (ii) a non-common conformation and composition of the S1 pocket; and (iii) the lack of the typical surface beta-ribbons together with a "featureless" shape of the substrate-binding site. Hydrolytic activity assays indicate that the S1 pocket recognises Glu and Asp side chains specifically, which, therefore, are predicted to occupy the P1 position on the substrate cleavage site. The positive electrostatic potential featured by the S1 region underlies this specificity. The comparative structural analysis highlights the peculiarity of the astrovirus protease, and differentiates it from the human and viral serine proteases.
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===STRUCTURAL AND BIOCHEMICAL ANALYSIS OF HUMAN PATHOGENIC ASTROVIRUS SERINE PROTEASE AT 2.0 ANGSTROM RESOLUTION===
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Structural and biochemical analysis of human pathogenic astrovirus serine protease at 2.0 A resolution.,Speroni S, Rohayem J, Nenci S, Bonivento D, Robel I, Barthel J, Luzhkov VB, Coutard B, Canard B, Mattevi A J Mol Biol. 2009 Apr 17;387(5):1137-52. Epub 2009 Feb 26. PMID:19249313<ref>PMID:19249313</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19249313}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2w5e" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19249313 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19249313}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2W5E is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Human_astrovirus_1 Human astrovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5E OCA].
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==Reference==
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<ref group="xtra">PMID:19249313</ref><references group="xtra"/>
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[[Category: Human astrovirus 1]]
[[Category: Human astrovirus 1]]
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[[Category: Barthel, J.]]
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[[Category: Large Structures]]
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[[Category: Bonivento, D.]]
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[[Category: Barthel J]]
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[[Category: Canard, B.]]
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[[Category: Bonivento D]]
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[[Category: Coutard, B.]]
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[[Category: Canard B]]
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[[Category: Mattevi, A.]]
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[[Category: Coutard B]]
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[[Category: Nenci, S.]]
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[[Category: Mattevi A]]
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[[Category: Robel, I.]]
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[[Category: Nenci S]]
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[[Category: Rohayem, J.]]
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[[Category: Robel I]]
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[[Category: Speroni, S.]]
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[[Category: Rohayem J]]
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[[Category: Antiviral]]
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[[Category: Speroni S]]
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[[Category: Astrovirus]]
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[[Category: Catalytic triad]]
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[[Category: Coiled coil]]
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[[Category: Hydrolase]]
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[[Category: Membrane]]
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[[Category: Protease]]
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[[Category: Ribosomal frameshifting]]
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[[Category: Rna replication]]
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[[Category: Serine protease]]
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[[Category: Thiol protease]]
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[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 11 11:17:15 2009''
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Current revision

Structural and biochemical analysis of human pathogenic astrovirus serine protease at 2.0 Angstrom resolution

PDB ID 2w5e

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