3czc

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{{Seed}}
 
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[[Image:3czc.jpg|left|200px]]
 
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==The Crystal Structure of a putative PTS IIB(PtxB) from Streptococcus mutans==
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The line below this paragraph, containing "STRUCTURE_3czc", creates the "Structure Box" on the page.
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<StructureSection load='3czc' size='340' side='right'caption='[[3czc]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3czc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CZC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3czc| PDB=3czc | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3czc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3czc OCA], [https://pdbe.org/3czc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3czc RCSB], [https://www.ebi.ac.uk/pdbsum/3czc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3czc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q93DB0_STRMG Q93DB0_STRMG]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cz/3czc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3czc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Streptococcus mutans is the primary etiological agent of dental caries in man and other mammalian organisms. This bacterium metabolizes carbohydrates actively and thrives under anaerobic conditions by fermenting l-ascorbate (Asc) via the sga operon, which includes SgaT, PtxB, and PtxA. These three proteins are members of the Asc family of enzyme II (EII) complexes of the bacterial phosphotransferase system. Here, we report the crystal structure of PtxB, solved by single-wavelength anomalous dispersion phasing, and that of PtxA, solved by molecular replacement, from S. mutans. PtxB provides the first crystal structure of an EIIB from the Asc family, composed of a central beta sheet of parallel strands flanked by alpha helices on both sides. The structure of PtxB is similar to the structures of IIB(Mtl) (IIB subunit of mannitol PTS) and IIB(Cel) (IIB subunit of cellobiose) in Escherichia coli despite the low sequence identity. PtxA adopts a globular alpha/beta sandwich structure. The phosphorylation-site His68 is situated between beta2 and beta3, within a hydrophobic pocket. We found that the hydrogen bond on N(delta1) of the active-site histidine is a common means of ensuring that phosphate is on the correct N(varepsilon2) site in many EIIA families. Finally, a model of the PtxB-PtxA complex was constructed, and a PtxA-phospho-PtxB state is proposed. Analyses of the two structures shed light on the catalytic mechanism of the phosphotransferase system.
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===The Crystal Structure of a putative PTS IIB(PtxB) from Streptococcus mutans===
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Crystal structures of phosphotransferase system enzymes PtxB (IIB(Asc)) and PtxA (IIA(Asc)) from Streptococcus mutans.,Lei J, Li LF, Su XD J Mol Biol. 2009 Feb 20;386(2):465-75. Epub 2008 Dec 25. PMID:19135450<ref>PMID:19135450</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19135450}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3czc" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19135450 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19135450}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3CZC is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CZC OCA].
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==Reference==
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<ref group="xtra">PMID:19135450</ref><references group="xtra"/>
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[[Category: Streptococcus mutans]]
[[Category: Streptococcus mutans]]
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[[Category: Lei, J.]]
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[[Category: Lei J]]
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[[Category: Su, X D.]]
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[[Category: Su XD]]
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[[Category: Alpha/beta sandwich]]
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[[Category: Phosphotransferase system]]
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[[Category: Transferase]]
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[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 11 11:22:09 2009''
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Current revision

The Crystal Structure of a putative PTS IIB(PtxB) from Streptococcus mutans

PDB ID 3czc

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