2nod

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(New page: 200px<br /><applet load="2nod" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nod, resolution 2.6&Aring;" /> '''MURINE INDUCIBLE NITR...)
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[[Image:2nod.jpg|left|200px]]<br /><applet load="2nod" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2nod, resolution 2.6&Aring;" />
 
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'''MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER (DELTA 65) WITH TETRAHYDROBIOPTERIN AND WATER BOUND IN ACTIVE CENTER'''<br />
 
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==Overview==
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==MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER (DELTA 65) WITH TETRAHYDROBIOPTERIN AND WATER BOUND IN ACTIVE CENTER==
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Crystal structures of the murine cytokine-inducible nitric oxide synthase, oxygenase dimer with active-center water molecules, the substrate, L-arginine (L-Arg), or product analog thiocitrulline reveal how, dimerization, cofactor tetrahydrobiopterin, and L-Arg binding complete the, catalytic center for synthesis of the essential biological signal and, cytotoxin nitric oxide. Pterin binding refolds the central interface, region, recruits new structural elements, creates a 30 angstrom deep, active-center channel, and causes a 35 degrees helical tilt to expose a, heme edge and the adjacent residue tryptophan-366 for likely reductase, domain interactions and caveolin inhibition. Heme propionate interactions, with pterin and L-Arg suggest that pterin has electronic influences on, heme-bound oxygen. L-Arginine binds to glutamic acid-371 and stacks with, heme in an otherwise hydrophobic pocket to aid activation of heme-bound, oxygen by direct proton donation and thereby differentiate the two, chemical steps of nitric oxide synthesis.
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<StructureSection load='2nod' size='340' side='right'caption='[[2nod]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nod]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NOD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nod OCA], [https://pdbe.org/2nod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nod RCSB], [https://www.ebi.ac.uk/pdbsum/2nod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nod ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NOS2_MOUSE NOS2_MOUSE] Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.<ref>PMID:16373578</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/no/2nod_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nod ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures of the murine cytokine-inducible nitric oxide synthase oxygenase dimer with active-center water molecules, the substrate L-arginine (L-Arg), or product analog thiocitrulline reveal how dimerization, cofactor tetrahydrobiopterin, and L-Arg binding complete the catalytic center for synthesis of the essential biological signal and cytotoxin nitric oxide. Pterin binding refolds the central interface region, recruits new structural elements, creates a 30 angstrom deep active-center channel, and causes a 35 degrees helical tilt to expose a heme edge and the adjacent residue tryptophan-366 for likely reductase domain interactions and caveolin inhibition. Heme propionate interactions with pterin and L-Arg suggest that pterin has electronic influences on heme-bound oxygen. L-Arginine binds to glutamic acid-371 and stacks with heme in an otherwise hydrophobic pocket to aid activation of heme-bound oxygen by direct proton donation and thereby differentiate the two chemical steps of nitric oxide synthesis.
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==About this Structure==
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Structure of nitric oxide synthase oxygenase dimer with pterin and substrate.,Crane BR, Arvai AS, Ghosh DK, Wu C, Getzoff ED, Stuehr DJ, Tainer JA Science. 1998 Mar 27;279(5359):2121-6. PMID:9516116<ref>PMID:9516116</ref>
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2NOD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4, HEM and H4B as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2NOD OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of nitric oxide synthase oxygenase dimer with pterin and substrate., Crane BR, Arvai AS, Ghosh DK, Wu C, Getzoff ED, Stuehr DJ, Tainer JA, Science. 1998 Mar 27;279(5359):2121-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9516116 9516116]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 2nod" style="background-color:#fffaf0;"></div>
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[[Category: Nitric-oxide synthase]]
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[[Category: Single protein]]
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[[Category: Arvai, A.S.]]
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[[Category: Crane, B.R.]]
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[[Category: Getzoff, E.D.]]
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[[Category: Stuehr, D.J.]]
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[[Category: Tainer, J.A.]]
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[[Category: H4B]]
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[[Category: HEM]]
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[[Category: SO4]]
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[[Category: dimer]]
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[[Category: heme]]
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[[Category: nitric oxide l-arginine monooxygenase]]
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[[Category: nos]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:48:51 2007''
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==See Also==
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*[[Nitric Oxide Synthase 3D structures|Nitric Oxide Synthase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Arvai AS]]
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[[Category: Crane BR]]
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[[Category: Getzoff ED]]
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[[Category: Stuehr DJ]]
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[[Category: Tainer JA]]

Current revision

MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER (DELTA 65) WITH TETRAHYDROBIOPTERIN AND WATER BOUND IN ACTIVE CENTER

PDB ID 2nod

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