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2nou

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(New page: 200px<br /><applet load="2nou" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nou" /> '''Membrane induced structure of Scyliorhinin I...)
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[[Image:2nou.gif|left|200px]]<br /><applet load="2nou" size="450" color="white" frame="true" align="right" spinBox="true"
 
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'''Membrane induced structure of Scyliorhinin I: A Dual NK1/NK2 agonist'''<br />
 
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==Overview==
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==Membrane induced structure of Scyliorhinin I: A Dual NK1/NK2 agonist==
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Scyliorhinin I, a linear decapeptide, is the only known tachykinin that, shows high affinity for both NK-1 and NK-2 binding sites and low affinity, for NK-3 binding sites. As a first step to understand the, structure-activity relationship, we report the membrane-induced structure, of scyliorhinin I with the aid of circular dichroism and 2D-(1)H NMR, spectroscopy. Sequence specific resonance assignments of protons have been, made from correlation spectroscopy (TOCSY, DQF-COSY) and NOESY, spectroscopy. The interproton distance constraints and dihedral angle, constraints have been utilized to generate a family of structures using, DYANA. The superimposition of 20 final structures has been reported with, backbone pairwise root mean-square deviation of 0.38 +/- 0.19 A. The, results show that scyliorhinin I exists in a random coil state in aqueous, environments, whereas helical conformation is induced toward the, C-terminal region of the peptide (D4-M10) in the presence of dodecyl, phosphocholine micelles. Analysis of NMR data is suggestive of the, presence of a 3(10)-helix that is in equilibrium with an alpha-helix in, this region from residue 4 to 10. An extended highly flexible N-terminus, of scyliorhinin I displays some degree of order and a possible turn, structure. Observed conformational features have been compared with, respect to that of substance P and neurokinin A, which are endogenous, agonists of NK-1 and NK-2 receptors, respectively.
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<StructureSection load='2nou' size='340' side='right'caption='[[2nou]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nou]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Scyliorhinus_canicula Scyliorhinus canicula]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NOU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nou OCA], [https://pdbe.org/2nou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nou RCSB], [https://www.ebi.ac.uk/pdbsum/2nou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nou ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TKN1_SCYCA TKN1_SCYCA] Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Scyliorhinin I, a linear decapeptide, is the only known tachykinin that shows high affinity for both NK-1 and NK-2 binding sites and low affinity for NK-3 binding sites. As a first step to understand the structure-activity relationship, we report the membrane-induced structure of scyliorhinin I with the aid of circular dichroism and 2D-(1)H NMR spectroscopy. Sequence specific resonance assignments of protons have been made from correlation spectroscopy (TOCSY, DQF-COSY) and NOESY spectroscopy. The interproton distance constraints and dihedral angle constraints have been utilized to generate a family of structures using DYANA. The superimposition of 20 final structures has been reported with backbone pairwise root mean-square deviation of 0.38 +/- 0.19 A. The results show that scyliorhinin I exists in a random coil state in aqueous environments, whereas helical conformation is induced toward the C-terminal region of the peptide (D4-M10) in the presence of dodecyl phosphocholine micelles. Analysis of NMR data is suggestive of the presence of a 3(10)-helix that is in equilibrium with an alpha-helix in this region from residue 4 to 10. An extended highly flexible N-terminus of scyliorhinin I displays some degree of order and a possible turn structure. Observed conformational features have been compared with respect to that of substance P and neurokinin A, which are endogenous agonists of NK-1 and NK-2 receptors, respectively.
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==About this Structure==
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Membrane-Induced Structure of Scyliorhinin I: A Dual NK1/NK2 Agonist.,Dike A, Cowsik SM Biophys J. 2005 May;88(5):3592-600. Epub 2005 Feb 24. PMID:15731392<ref>PMID:15731392</ref>
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2NOU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2NOU OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Membrane-Induced Structure of Scyliorhinin I: A Dual NK1/NK2 Agonist., Dike A, Cowsik SM, Biophys J. 2005 May;88(5):3592-600. Epub 2005 Feb 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15731392 15731392]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2nou" style="background-color:#fffaf0;"></div>
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[[Category: Cowsik, S.M.]]
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== References ==
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[[Category: Dike, A.]]
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<references/>
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[[Category: 3-10 helix]]
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__TOC__
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[[Category: dpc micelles]]
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</StructureSection>
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[[Category: helix]]
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[[Category: Large Structures]]
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[[Category: lipid induced conformation]]
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[[Category: Scyliorhinus canicula]]
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[[Category: Cowsik SM]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:49:17 2007''
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[[Category: Dike A]]

Current revision

Membrane induced structure of Scyliorhinin I: A Dual NK1/NK2 agonist

PDB ID 2nou

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