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2ra5

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{{Seed}}
 
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[[Image:2ra5.png|left|200px]]
 
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==Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor==
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The line below this paragraph, containing "STRUCTURE_2ra5", creates the "Structure Box" on the page.
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<StructureSection load='2ra5' size='340' side='right'caption='[[2ra5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ra5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RA5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
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-->
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2ra5| PDB=2ra5 | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCO6256 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ra5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ra5 OCA], [https://pdbe.org/2ra5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ra5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ra5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ra5 ProSAT], [https://www.topsan.org/Proteins/MCSG/2ra5 TOPSAN]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2ra5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ra5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
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===Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor===
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In situ proteolysis for protein crystallization and structure determination.,Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461<ref>PMID:17982461</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17982461}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2ra5" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17982461 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_17982461}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2RA5 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RA5 OCA].
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[[Category: Strco]]
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[[Category: Edwards, A M]]
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==Reference==
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[[Category: Joachimiak, A]]
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<ref group="xtra">PMID:17982461</ref><references group="xtra"/>
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[[Category: Kim, Y]]
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[[Category: Bacteria]]
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[[Category: Structural genomic]]
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[[Category: Edwards, A M.]]
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[[Category: Savchenko, A]]
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[[Category: Joachimiak, A.]]
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[[Category: Xu, X]]
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[[Category: Kim, Y.]]
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[[Category: Zheng, H]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Savchenko, A.]]
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[[Category: Xu, X.]]
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[[Category: Zheng, H.]]
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[[Category: Beta structure]]
[[Category: Beta structure]]
[[Category: Dna-binding]]
[[Category: Dna-binding]]
[[Category: Mcsg]]
[[Category: Mcsg]]
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[[Category: Midwest center for structural genomic]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Structural genomic]]
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[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
[[Category: Utra domain]]
[[Category: Utra domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 10:26:58 2009''
 

Current revision

Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor

PDB ID 2ra5

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