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2nuu

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(New page: 200px<br /><applet load="2nuu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nuu, resolution 2.50&Aring;" /> '''Regulating the Esche...)
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[[Image:2nuu.gif|left|200px]]<br /><applet load="2nuu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2nuu, resolution 2.50&Aring;" />
 
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'''Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex'''<br />
 
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==Overview==
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==Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex==
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Amt proteins are ubiquitous channels for the conduction of ammonia in, archaea, eubacteria, fungi, and plants. In Escherichia coli, previous, studies have indicated that binding of the PII signal transduction protein, GlnK to the ammonia channel AmtB regulates the channel thereby controlling, ammonium influx in response to the intracellular nitrogen status. Here, we, describe the crystal structure of the complex between AmtB and GlnK at a, resolution of 2.5 A. This structure of PII in a complex with one of its, targets reveals physiologically relevant conformations of both AmtB and, GlnK. GlnK interacts with AmtB almost exclusively via a long surface loop, containing Y51 (T-loop), the tip of which inserts deeply into the, cytoplasmic pore exit, blocking ammonia conduction. Y51 of GlnK is also, buried in the pore exit, explaining why uridylylation of this residue, prevents complex formation.
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<StructureSection load='2nuu' size='340' side='right'caption='[[2nuu]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nuu]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NUU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nuu OCA], [https://pdbe.org/2nuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nuu RCSB], [https://www.ebi.ac.uk/pdbsum/2nuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nuu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI] Involved in the uptake of ammonia.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/2nuu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nuu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Amt proteins are ubiquitous channels for the conduction of ammonia in archaea, eubacteria, fungi, and plants. In Escherichia coli, previous studies have indicated that binding of the PII signal transduction protein GlnK to the ammonia channel AmtB regulates the channel thereby controlling ammonium influx in response to the intracellular nitrogen status. Here, we describe the crystal structure of the complex between AmtB and GlnK at a resolution of 2.5 A. This structure of PII in a complex with one of its targets reveals physiologically relevant conformations of both AmtB and GlnK. GlnK interacts with AmtB almost exclusively via a long surface loop containing Y51 (T-loop), the tip of which inserts deeply into the cytoplasmic pore exit, blocking ammonia conduction. Y51 of GlnK is also buried in the pore exit, explaining why uridylylation of this residue prevents complex formation.
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==About this Structure==
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The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.,Conroy MJ, Durand A, Lupo D, Li XD, Bullough PA, Winkler FK, Merrick M Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1213-8. Epub 2007 Jan 12. PMID:17220269<ref>PMID:17220269</ref>
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2NUU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2NUU OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel., Conroy MJ, Durand A, Lupo D, Li XD, Bullough PA, Winkler FK, Merrick M, Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1213-8. Epub 2007 Jan 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17220269 17220269]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 2nuu" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Bullough, P.A.]]
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[[Category: Conroy, M.J.]]
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[[Category: Durand, A.]]
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[[Category: Li, X.D.]]
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[[Category: Lupo, D.]]
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[[Category: Merrick, M.]]
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[[Category: Winkler, F.K.]]
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[[Category: ADP]]
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[[Category: ammonia transport]]
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[[Category: membrane protein complex]]
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[[Category: nitrogen regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:55:35 2007''
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==See Also==
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Bullough PA]]
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[[Category: Conroy MJ]]
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[[Category: Durand A]]
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[[Category: Li X-D]]
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[[Category: Lupo D]]
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[[Category: Merrick M]]
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[[Category: Winkler FK]]

Current revision

Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex

PDB ID 2nuu

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