2i3e

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{{Seed}}
 
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[[Image:2i3e.png|left|200px]]
 
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==Solution structure of catalytic domain of goldfish RICH protein==
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The line below this paragraph, containing "STRUCTURE_2i3e", creates the "Structure Box" on the page.
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<StructureSection load='2i3e' size='340' side='right'caption='[[2i3e]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2i3e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carassius_auratus Carassius auratus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I3E FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i3e OCA], [https://pdbe.org/2i3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i3e RCSB], [https://www.ebi.ac.uk/pdbsum/2i3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i3e ProSAT]</span></td></tr>
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{{STRUCTURE_2i3e| PDB=2i3e | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q90306_CARAU Q90306_CARAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i3/2i3e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i3e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Regeneration-induced CNPase homolog (RICH) is an axonal growth-associated protein, which is induced in teleost fish upon optical nerve injury. RICH consists of a highly acidic N-terminal domain, a catalytic domain with 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) activity and a C-terminal isoprenylation site. In vitro RICH and mammalian brain CNPase specifically catalyze the hydrolysis of 2',3'-cyclic nucleotides to produce 2'-nucleotides, but the physiologically relevant in vivo substrate remains unknown. Here, we report the NMR structure of the catalytic domain of goldfish RICH and describe its binding to CNPase inhibitors. The structure consists of a twisted nine-stranded antiparallel beta-sheet surrounded by alpha-helices on both sides. Despite significant local differences mostly arising from a seven-residue insert in the RICH sequence, the active site region is highly similar to that of human CNPase. Likewise, refinement of the catalytic domain of rat CNPase using residual dipolar couplings gave improved agreement with the published crystal structure. NMR titrations of RICH with inhibitors point to a similar catalytic mechanism for RICH and CNPase. The results suggest a functional importance for the evolutionarily conserved phosphodiesterase activity and hint of a link with pre-tRNA splicing.
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===Solution structure of catalytic domain of goldfish RICH protein===
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Solution structure of the catalytic domain of RICH protein from goldfish.,Kozlov G, Denisov AY, Pomerantseva E, Gravel M, Braun PE, Gehring K FEBS J. 2007 Mar;274(6):1600-9. PMID:17480208<ref>PMID:17480208</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17480208}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2i3e" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17480208 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17480208}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2I3E is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Carassius_auratus Carassius auratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I3E OCA].
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==Reference==
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<ref group="xtra">PMID:17480208</ref><references group="xtra"/>
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[[Category: 2',3'-cyclic-nucleotide 3'-phosphodiesterase]]
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[[Category: Carassius auratus]]
[[Category: Carassius auratus]]
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[[Category: Denisov, A Y.]]
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[[Category: Large Structures]]
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[[Category: Gehring, K.]]
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[[Category: Denisov AY]]
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[[Category: Kozlov, G.]]
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[[Category: Gehring K]]
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[[Category: 2s']]
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[[Category: Kozlov G]]
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[[Category: 3'-cyclic-nucleotide 3'-phosphodiesterase]]
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[[Category: Cnp]]
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[[Category: Hydrolase]]
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[[Category: Nerve regeneration]]
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[[Category: Rich protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 10:55:36 2009''
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Current revision

Solution structure of catalytic domain of goldfish RICH protein

PDB ID 2i3e

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