2nv6

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(New page: 200px<br /><applet load="2nv6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nv6, resolution 1.90&Aring;" /> '''Mycobacterium tuberc...)
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[[Image:2nv6.gif|left|200px]]<br /><applet load="2nv6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2nv6, resolution 1.90&Aring;" />
 
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'''Mycobacterium tuberculosis InhA (S94A) bound with INH-NAD adduct'''<br />
 
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==Overview==
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==Mycobacterium tuberculosis InhA (S94A) bound with INH-NAD adduct==
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Isoniazid is one of the most effective antituberculosis drugs, yet its, precise mechanism of action is still controversial. Using specialized, linkage transduction, a single point mutation allele (S94A) within the, putative target gene inhA was transferred in Mycobacterium tuberculosis., The inhA(S94A) allele was sufficient to confer clinically relevant levels, of resistance to isoniazid killing and inhibition of mycolic acid, biosynthesis. This resistance correlated with the decreased binding of the, INH-NAD inhibitor to InhA, as shown by enzymatic and X-ray, crystallographic analyses, and establishes InhA as the primary target of, isoniazid action in M. tuberculosis.
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<StructureSection load='2nv6' size='340' side='right'caption='[[2nv6]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nv6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NV6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZID:ISONICOTINIC-ACETYL-NICOTINAMIDE-ADENINE+DINUCLEOTIDE'>ZID</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nv6 OCA], [https://pdbe.org/2nv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nv6 RCSB], [https://www.ebi.ac.uk/pdbsum/2nv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nv6 ProSAT], [https://www.topsan.org/Proteins/TBSGC/2nv6 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/INHA_MYCTU INHA_MYCTU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nv/2nv6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nv6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Isoniazid is one of the most effective antituberculosis drugs, yet its precise mechanism of action is still controversial. Using specialized linkage transduction, a single point mutation allele (S94A) within the putative target gene inhA was transferred in Mycobacterium tuberculosis. The inhA(S94A) allele was sufficient to confer clinically relevant levels of resistance to isoniazid killing and inhibition of mycolic acid biosynthesis. This resistance correlated with the decreased binding of the INH-NAD inhibitor to InhA, as shown by enzymatic and X-ray crystallographic analyses, and establishes InhA as the primary target of isoniazid action in M. tuberculosis.
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==About this Structure==
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Transfer of a point mutation in Mycobacterium tuberculosis inhA resolves the target of isoniazid.,Vilcheze C, Wang F, Arai M, Hazbon MH, Colangeli R, Kremer L, Weisbrod TR, Alland D, Sacchettini JC, Jacobs WR Jr Nat Med. 2006 Sep;12(9):1027-9. Epub 2006 Aug 13. PMID:16906155<ref>PMID:16906155</ref>
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2NV6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with ZID as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2NV6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Transfer of a point mutation in Mycobacterium tuberculosis inhA resolves the target of isoniazid., Vilcheze C, Wang F, Arai M, Hazbon MH, Colangeli R, Kremer L, Weisbrod TR, Alland D, Sacchettini JC, Jacobs WR Jr, Nat Med. 2006 Sep;12(9):1027-9. Epub 2006 Aug 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16906155 16906155]
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</div>
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[[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
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<div class="pdbe-citations 2nv6" style="background-color:#fffaf0;"></div>
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Sacchettini, J.C.]]
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[[Category: Wang, F.]]
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[[Category: ZID]]
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[[Category: inha]]
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[[Category: isoniazid]]
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[[Category: s94a]]
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[[Category: tuberculosis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:55:57 2007''
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==See Also==
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*[[Enoyl-Acyl-Carrier Protein Reductase 3D structures|Enoyl-Acyl-Carrier Protein Reductase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Sacchettini JC]]
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[[Category: Wang F]]

Current revision

Mycobacterium tuberculosis InhA (S94A) bound with INH-NAD adduct

PDB ID 2nv6

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