3cva

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{{Seed}}
 
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[[Image:3cva.jpg|left|200px]]
 
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==Human Bcl-xL containing a Trp to Ala mutation at position 137==
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The line below this paragraph, containing "STRUCTURE_3cva", creates the "Structure Box" on the page.
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<StructureSection load='3cva' size='340' side='right'caption='[[3cva]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cva]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CVA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cva OCA], [https://pdbe.org/3cva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cva RCSB], [https://www.ebi.ac.uk/pdbsum/3cva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cva ProSAT]</span></td></tr>
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{{STRUCTURE_3cva| PDB=3cva | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cv/3cva_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cva ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bcl-2 family proteins regulate apoptosis through their homo- and heterodimerization. By protein sequence analysis and structural comparison, we have identified a conserved hydrophobic core at the BH1 and BH2 domains of Bcl-2 family proteins. The hydrophobic core is stabilized by hydrophobic interactions among the residues of Trp137, Ile140, Trp181, Ile182, Trp188 and Phe191 in Bcl-x(L). Destabilization of the hydrophobic core can promote the protein unfolding and pore formation in synthetic lipid vesicles. Interestingly, though the hydrophobic core does not participate in binding with BH3 domain of pro-apoptotic proteins, disruption of the hydrophobic core can reduce the affinity of Bcl-x(L) with BH3-domain peptide by changing the conformation of Bcl-x(L) C-terminal residues that are involved in the peptide interaction. The BH3-domain peptide binding affinity and pore forming propensity of Bcl-x(L) were correlated to its death-repressor activity, which provides new information to help study the regulatory mechanism of anti-apoptotic proteins. Meanwhile, as the tryptophans are conserved in the hydrophobic core, in vitro binding assay based on FRET of "Trp--&gt;AEDANS" can be devised to screen for new modulators targeting anti-apoptotic proteins as well as "multi-BH domains" pro-apoptotic proteins.
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===Human Bcl-xL containing a Trp to Ala mutation at position 137===
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A conserved hydrophobic core at Bcl-xL mediates its structural stability and binding affinity with BH3-domain peptide of pro-apoptotic protein.,Feng Y, Zhang L, Hu T, Shen X, Ding J, Chen K, Jiang H, Liu D Arch Biochem Biophys. 2009 Apr 1;484(1):46-54. Epub 2009 Jan 10. PMID:19161970<ref>PMID:19161970</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3cva" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19161970}}, adds the Publication Abstract to the page
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*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19161970 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19161970}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3CVA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CVA OCA].
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==Reference==
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<ref group="xtra">PMID:19161970</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Chen, K.]]
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[[Category: Large Structures]]
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[[Category: Feng, Y.]]
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[[Category: Chen K]]
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[[Category: Hu, T.]]
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[[Category: Feng Y]]
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[[Category: Jiang, H.]]
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[[Category: Hu T]]
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[[Category: Liu, D.]]
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[[Category: Jiang H]]
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[[Category: Shen, X.]]
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[[Category: Liu D]]
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[[Category: Zhang, L.]]
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[[Category: Shen X]]
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[[Category: Alternative splicing]]
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[[Category: Zhang L]]
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[[Category: Apoptosis]]
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[[Category: Membrane]]
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[[Category: Mitochondrion]]
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[[Category: Nucleus]]
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[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 12:24:04 2009''
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Current revision

Human Bcl-xL containing a Trp to Ala mutation at position 137

PDB ID 3cva

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OCA

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