3die

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{{Seed}}
 
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[[Image:3die.jpg|left|200px]]
 
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==Domain swapping of Staphylococcus Aureus thioredoxin W28A mutant==
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The line below this paragraph, containing "STRUCTURE_3die", creates the "Structure Box" on the page.
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<StructureSection load='3die' size='340' side='right'caption='[[3die]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3die]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DIE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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{{STRUCTURE_3die| PDB=3die | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3die FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3die OCA], [https://pdbe.org/3die PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3die RCSB], [https://www.ebi.ac.uk/pdbsum/3die PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3die ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/THIO_STAAU THIO_STAAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/di/3die_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3die ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The thioredoxin (Trx) fold is a small monomeric domain that is ubiquitous in redox-active enzymes. Trxs are characterized by a typical WCGPC active-site sequence motif. A single active-site mutation of the tryptophan to an alanine in Staphylococcus aureus Trx converts the oxidized protein into a biologically inactive domain-swapped dimer. While the monomeric protein unfolds reversibly in a two-state manner, the oxidized dimeric form is kinetically stable and converts to the monomeric form upon refolding. After reduction, the half-life of the dimer decreases many orders of magnitude to approximately 4.3 h, indicating that the active-site disulfide between Cys29 and Cys32 is an important determinant for the kinetics of unfolding. We propose kinetic stability as a possible evolutionary strategy in the evolution of multimeric proteins from their monomeric ancestors by domain swapping, which, for this biologically inactive Trx mutant, turned out to be an evolutionary dead end.
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===Domain swapping of Staphylococcus Aureus thioredoxin W28A mutant===
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Coupling of domain swapping to kinetic stability in a thioredoxin mutant.,Garcia-Pino A, Martinez-Rodriguez S, Wahni K, Wyns L, Loris R, Messens J J Mol Biol. 2009 Feb 6;385(5):1590-9. Epub 2008 Dec 3. PMID:19071139<ref>PMID:19071139</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3die" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19071139}}, adds the Publication Abstract to the page
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*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19071139 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19071139}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3DIE is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIE OCA].
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==Reference==
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<ref group="xtra">PMID:19071139</ref><references group="xtra"/>
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Loris, R.]]
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[[Category: Loris R]]
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[[Category: Martinez-Rodriguez, S.]]
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[[Category: Martinez-Rodriguez S]]
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[[Category: Messens, J.]]
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[[Category: Messens J]]
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[[Category: Electron transport]]
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[[Category: Oxidoreductase]]
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[[Category: Redox enzymology]]
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[[Category: Redox-active center]]
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[[Category: Swap domain]]
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[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 12:24:44 2009''
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Current revision

Domain swapping of Staphylococcus Aureus thioredoxin W28A mutant

PDB ID 3die

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