3ftf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:08, 30 August 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3ftf.jpg|left|200px]]
 
-
<!--
+
==Crystal structure of A. aeolicus KsgA in complex with RNA and SAH==
-
The line below this paragraph, containing "STRUCTURE_3ftf", creates the "Structure Box" on the page.
+
<StructureSection load='3ftf' size='340' side='right'caption='[[3ftf]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3ftf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FTF FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-
{{STRUCTURE_3ftf| PDB=3ftf | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ftf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ftf OCA], [https://pdbe.org/3ftf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ftf RCSB], [https://www.ebi.ac.uk/pdbsum/3ftf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ftf ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RSMA_AQUAE RSMA_AQUAE] Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/3ftf_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ftf ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Among methyltransferases, KsgA and the reaction it catalyzes are conserved throughout evolution. However, the specifics of substrate recognition by the enzyme remain unknown. Here we report structures of Aquifex aeolicus KsgA, in its ligand-free form, in complex with RNA, and in complex with both RNA and S-adenosylhomocysteine (SAH, reaction product of cofactor S-adenosylmethionine), revealing critical structural information on KsgA-RNA and KsgA-SAH interactions. Moreover, the structures show how conformational changes that occur upon RNA binding create the cofactor-binding site. There are nine conserved functional motifs (motifs I-VIII and X) in KsgA. Prior to RNA binding, motifs I and VIII are flexible, each exhibiting two distinct conformations. Upon RNA binding, the two motifs become stabilized in one of these conformations, which is compatible with the binding of SAH. Motif X, which is also stabilized upon RNA binding, is directly involved in the binding of SAH.
-
===Crystal structure of A. aeolicus KsgA in complex with RNA and SAH===
+
Structural basis for binding of RNA and cofactor by a KsgA methyltransferase.,Tu C, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X Structure. 2009 Mar 11;17(3):374-85. PMID:19278652<ref>PMID:19278652</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3ftf" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_19278652}}, adds the Publication Abstract to the page
+
*[[Adenosine dimethyltransferase|Adenosine dimethyltransferase]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 19278652 is the PubMed ID number.
+
*[[Adenosine dimethyltransferase 3D structures|Adenosine dimethyltransferase 3D structures]]
-
-->
+
== References ==
-
{{ABSTRACT_PUBMED_19278652}}
+
<references/>
-
 
+
__TOC__
-
==About this Structure==
+
</StructureSection>
-
3FTF is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FTF OCA].
+
[[Category: Aquifex aeolicus VF5]]
-
 
+
[[Category: Large Structures]]
-
==Reference==
+
[[Category: Ji X]]
-
<ref group="xtra">PMID:19278652</ref><references group="xtra"/>
+
[[Category: Tu C]]
-
[[Category: Aquifex aeolicus vf5]]
+
-
[[Category: Ji, X.]]
+
-
[[Category: Tu, C.]]
+
-
[[Category: Antibiotic resistance]]
+
-
[[Category: Ksga]]
+
-
[[Category: Methyltransferase]]
+
-
[[Category: Mtase]]
+
-
[[Category: Rna]]
+
-
[[Category: Rna methyltransferase]]
+
-
[[Category: Rna-binding]]
+
-
[[Category: Rossmann-like fold]]
+
-
[[Category: Rrna processing]]
+
-
[[Category: S-adenosyl-l-methionine]]
+
-
[[Category: Transferase]]
+
-
[[Category: Transferase/rna complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 12:31:17 2009''
+

Current revision

Crystal structure of A. aeolicus KsgA in complex with RNA and SAH

PDB ID 3ftf

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ftf"
Personal tools