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3gj2

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[[Image:3gj2.jpg|left|200px]]
 
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==Photoactivated state of PA-GFP==
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The line below this paragraph, containing "STRUCTURE_3gj2", creates the "Structure Box" on the page.
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<StructureSection load='3gj2' size='340' side='right'caption='[[3gj2]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3gj2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GJ2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
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{{STRUCTURE_3gj2| PDB=3gj2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gj2 OCA], [https://pdbe.org/3gj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gj2 RCSB], [https://www.ebi.ac.uk/pdbsum/3gj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gj2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/3gj2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gj2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures of the photoactivatable green fluorescent protein T203H variant (PA-GFP) have been solved in the native and photoactivated states, which under 488 nm illumination are dark and brightly fluorescent, respectively. We demonstrate that photoactivation of PA-GFP is the result of a UV-induced decarboxylation of the Glu222 side chain that shifts the chromophore equilibrium to the anionic form. Coupled with the T203H mutation, which stabilizes the native PA-GFP neutral chromophore, Glu222 decarboxylation yields a 100-fold contrast enhancement relative to wild-type GFP (WT). Additionally, the structures provide insights into the spectroscopic differences between WT and PA-GFP steady-state fluorescence maxima and excited-state proton transfer dynamics.
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===Photoactivated state of PA-GFP===
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Structure and Mechanism of the Photoactivatable Green Fluorescent Protein.,Henderson JN, Gepshtein R, Heenan JR, Kallio K, Huppert D, Remington SJ J Am Chem Soc. 2009 Mar 11. PMID:19278226<ref>PMID:19278226</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3gj2" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19278226 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19278226}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3GJ2 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GJ2 OCA].
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==Reference==
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<ref group="xtra">PMID:19278226</ref><references group="xtra"/>
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Gepshtein, R.]]
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[[Category: Large Structures]]
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[[Category: Heenan, J R.]]
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[[Category: Gepshtein R]]
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[[Category: Henderson, J N.]]
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[[Category: Heenan JR]]
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[[Category: Huppert, D.]]
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[[Category: Henderson JN]]
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[[Category: Kallio, K.]]
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[[Category: Huppert D]]
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[[Category: Remington, S J.]]
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[[Category: Kallio K]]
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[[Category: Beta barrel]]
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[[Category: Remington SJ]]
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[[Category: Chromophore]]
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[[Category: Luminescence]]
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[[Category: Luminescent protein]]
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[[Category: Photoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 12:40:33 2009''
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Current revision

Photoactivated state of PA-GFP

PDB ID 3gj2

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