2w3o

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{{Seed}}
 
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[[Image:2w3o.png|left|200px]]
 
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==Crystal structure of the human PNKP FHA domain in complex with an XRCC1-derived phosphopeptide==
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The line below this paragraph, containing "STRUCTURE_2w3o", creates the "Structure Box" on the page.
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<StructureSection load='2w3o' size='340' side='right'caption='[[2w3o]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w3o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W3O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
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{{STRUCTURE_2w3o| PDB=2w3o | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w3o OCA], [https://pdbe.org/2w3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w3o RCSB], [https://www.ebi.ac.uk/pdbsum/2w3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w3o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XRCC1_HUMAN XRCC1_HUMAN] Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w3/2w3o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w3o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Short-patch repair of DNA single-strand breaks and gaps (SSB) is coordinated by XRCC1, a scaffold protein that recruits the DNA polymerase and DNA ligase required for filling and sealing the damaged strand. XRCC1 can also recruit end-processing enzymes, such as PNK (polynucleotide kinase 3'-phosphatase), Aprataxin and APLF (aprataxin/PNK-like factor), which ensure the availability of a free 3'-hydroxyl on one side of the gap, and a 5'-phosphate group on the other, for the polymerase and ligase reactions respectively. PNK binds to a phosphorylated segment of XRCC1 (between its two C-terminal BRCT domains) via its Forkhead-associated (FHA) domain. We show here, contrary to previous studies, that the FHA domain of PNK binds specifically, and with high affinity to a multiply phosphorylated motif in XRCC1 containing a pSer-pThr dipeptide, and forms a 2:1 PNK:XRCC1 complex. The high-resolution crystal structure of a PNK-FHA-XRCC1 phosphopeptide complex reveals the basis for this unusual bis-phosphopeptide recognition, which is probably a common feature of the known XRCC1-associating end-processing enzymes.
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===CRYSTAL STRUCTURE OF THE HUMAN PNKP FHA DOMAIN IN COMPLEX WITH AN XRCC1-DERIVED PHOSPHOPEPTIDE===
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Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK.,Ali AA, Jukes RM, Pearl LH, Oliver AW Nucleic Acids Res. 2009 Jan 20. PMID:19155274<ref>PMID:19155274</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19155274}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2w3o" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19155274 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19155274}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2W3O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W3O OCA].
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==Reference==
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Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK., Ali AA, Jukes RM, Pearl LH, Oliver AW, Nucleic Acids Res. 2009 Jan 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19155274 19155274]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Ali AAE]]
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[[Category: Atp-binding]]
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[[Category: Oliver AW]]
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[[Category: Base excision repair]]
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[[Category: Pearl LH]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Fha]]
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[[Category: Hydrolase]]
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[[Category: Kinase]]
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[[Category: Multifunctional enzyme]]
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[[Category: Nucleotide-binding]]
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[[Category: Nucleus]]
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[[Category: Phospho- peptide]]
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[[Category: Phosphoprotein]]
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[[Category: Pnkp]]
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[[Category: Polymorphism]]
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[[Category: Polynucleotide kinase 3' phosphatase]]
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[[Category: Transferase]]
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[[Category: Transferase/peptide]]
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[[Category: Xrcc1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 20:33:50 2009''
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Current revision

Crystal structure of the human PNKP FHA domain in complex with an XRCC1-derived phosphopeptide

PDB ID 2w3o

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