3foz

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{{Seed}}
 
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[[Image:3foz.png|left|200px]]
 
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==Structure of E. coli Isopentenyl-tRNA transferase in complex with E. coli tRNA(Phe)==
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The line below this paragraph, containing "STRUCTURE_3foz", creates the "Structure Box" on the page.
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<StructureSection load='3foz' size='340' side='right'caption='[[3foz]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3foz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_3foz| PDB=3foz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3foz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3foz OCA], [https://pdbe.org/3foz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3foz RCSB], [https://www.ebi.ac.uk/pdbsum/3foz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3foz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MIAA_ECOLI MIAA_ECOLI] Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).<ref>PMID:9012675</ref> <ref>PMID:9148919</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/3foz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3foz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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tRNAs that read codons starting with U are usually modified at their A37 by isopentenyl-tRNA transferases in order to minimize peptidyl-tRNA slippage in translation. The consensus substrate requirements of E. coli's isopentenyl-tRNA transferase, MiaA, have been the focus of extensive study. However, the molecular basis of tRNA-MiaA recognition remains unknown. Here, we describe the 2.5 A crystal structure of MiaA in complex with substrate tRNA(Phe). Comparative structural analysis reveals that the enzymatic reaction involves an RNA-protein mutually induced fit mechanism in which large domain movements in MiaA provoke the partial unfolding of the substrate tRNA anticodon loop. In addition, we show how substrate tRNAs are recognized by MiaA through a combination of direct and indirect sequence readouts.
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===Structure of E. coli Isopentenyl-tRNA transferase in complex with E. coli tRNA(Phe)===
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RNA-protein mutually induced Fit: Structure of E. coli isopentenyl-tRNA transferase in complex with tRNA(Phe).,Seif E, Hallberg BM J Biol Chem. 2009 Jan 21. PMID:19158097<ref>PMID:19158097</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3foz" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19158097}}, adds the Publication Abstract to the page
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19158097 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19158097}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli]]
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3FOZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOZ OCA].
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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==Reference==
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[[Category: Hallberg BM]]
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RNA-protein mutually induced Fit: Structure of E. coli isopentenyl-tRNA transferase in complex with tRNA(Phe)., Seif E, Hallberg BM, J Biol Chem. 2009 Jan 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19158097 19158097]
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[[Category: Seif E]]
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[[Category: Escherichia coli k-12]]
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[[Category: Protein complex]]
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[[Category: TRNA isopentenyltransferase]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Isopentenyl-trna transferase]]
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[[Category: Miaa]]
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[[Category: Nucleoside modification]]
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[[Category: Transferase/rna complex]]
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[[Category: Trna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 21:09:49 2009''
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Current revision

Structure of E. coli Isopentenyl-tRNA transferase in complex with E. coli tRNA(Phe)

PDB ID 3foz

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