3fto

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{{Seed}}
 
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[[Image:3fto.jpg|left|200px]]
 
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==Crystal structure of OppA in a open conformation==
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The line below this paragraph, containing "STRUCTURE_3fto", creates the "Structure Box" on the page.
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<StructureSection load='3fto' size='340' side='right'caption='[[3fto]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fto]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_cremoris Lactococcus cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FTO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3fto| PDB=3fto | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fto OCA], [https://pdbe.org/3fto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fto RCSB], [https://www.ebi.ac.uk/pdbsum/3fto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fto ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/3fto_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fto ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lactococcus lactis is a promising host for (membrane) protein overproduction. Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in L. lactis. Incorporation efficiencies of SeMet in the membrane protein complex OpuA (an ABC transporter) and the soluble protein OppA, both from L. lactis, were monitored by mass spectrometry. Both proteins incorporated SeMet with high efficiencies (&gt;90%), which greatly extends the usefulness of the expression host L. lactis for X-ray crystallography purposes. The crystal structure of ligand-free OppA was determined at 2.4 A resolution by a semiautomatic approach using selenium single-wavelength anomalous diffraction phasing.
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===Crystal structure of OppA in a open conformation===
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Selenomethionine incorporation in proteins expressed in Lactococcus lactis.,Berntsson RP, Alia Oktaviani N, Fusetti F, Thunnissen AM, Poolman B, Slotboom DJ Protein Sci. 2009 May;18(5):1121-7. PMID:19388077<ref>PMID:19388077</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3fto" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3FTO is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris Lactococcus lactis subsp. cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FTO OCA].
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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[[Category: Lactococcus lactis subsp. cremoris]]
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== References ==
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[[Category: Berntsson, R P.A.]]
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<references/>
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[[Category: Fusetti, F.]]
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__TOC__
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[[Category: Oktaviani, N A.]]
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</StructureSection>
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[[Category: Poolman, B.]]
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[[Category: Lactococcus cremoris]]
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[[Category: Slotboom, D J.]]
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[[Category: Large Structures]]
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[[Category: Thunnissen, A M.W H.]]
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[[Category: Berntsson RP-A]]
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[[Category: Oligo-peptide binding]]
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[[Category: Fusetti F]]
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[[Category: Peptide binding protein]]
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[[Category: Oktaviani NA]]
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[[Category: Venus fly-trap]]
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[[Category: Poolman B]]
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[[Category: Voluminous binding cavity]]
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[[Category: Slotboom D-J]]
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[[Category: Thunnissen A-MWH]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 2 16:12:21 2009''
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Current revision

Crystal structure of OppA in a open conformation

PDB ID 3fto

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