3gcn
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:3gcn.jpg|left|200px]] | ||
| - | + | ==Crystal structure of DegS H198P/D320A mutant modified by DFP in complex with OMP peptide (YQF)== | |
| - | + | <StructureSection load='3gcn' size='340' side='right'caption='[[3gcn]], [[Resolution|resolution]] 3.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3gcn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GCN FirstGlance]. <br> | |
| - | + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MIS:MONOISOPROPYLPHOSPHORYLSERINE'>MIS</scene></td></tr> | |
| - | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gco|3gco]], [[3gds|3gds]], [[3gdu|3gdu]], [[3gdv|3gdv]]</div></td></tr> | |
| - | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b3235, degS, hhoB, htrH, JW3204 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gcn OCA], [https://pdbe.org/3gcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gcn RCSB], [https://www.ebi.ac.uk/pdbsum/3gcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gcn ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| - | + | [[https://www.uniprot.org/uniprot/DEGS_ECOLI DEGS_ECOLI]] When heat shock or other environmental stresses disrupt protein folding in the periplasm, DegS senses the accumulation of unassembled outer membrane porins (OMPs) and then initiates RseA (anti sigma-E factor) degradation by cleaving it in its periplasmic domain, making it an attractive substrate for subsequent cleavage by RseP. This cascade that ultimately leads to the sigma-E-driven expression of a variety of factors dealing with folding stress in the periplasm and OMP assembly.<ref>PMID:12183369</ref> <ref>PMID:19695325</ref> | |
| - | == | + | == Evolutionary Conservation == |
| - | + | [[Image:Consurf_key_small.gif|200px|right]] | |
| - | [[ | + | Check<jmol> |
| - | [[Category: Grant, R A | + | <jmolCheckbox> |
| - | [[Category: Sauer, R T | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/3gcn_consurf.spt"</scriptWhenChecked> |
| - | [[Category: Sohn, J | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gcn ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Ecoli]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Grant, R A]] | ||
| + | [[Category: Sauer, R T]] | ||
| + | [[Category: Sohn, J]] | ||
[[Category: Htra]] | [[Category: Htra]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
| - | [[Category: Hydrolase | + | [[Category: Hydrolase-hydrolase activator complex]] |
[[Category: Omp]] | [[Category: Omp]] | ||
[[Category: Pdz domain]] | [[Category: Pdz domain]] | ||
| - | [[Category: Periplasm]] | ||
[[Category: Protease]] | [[Category: Protease]] | ||
[[Category: Serine protease]] | [[Category: Serine protease]] | ||
[[Category: Stress-sensor]] | [[Category: Stress-sensor]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 2 16:13:44 2009'' | ||
Current revision
Crystal structure of DegS H198P/D320A mutant modified by DFP in complex with OMP peptide (YQF)
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