2or1

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(New page: 200px<br /><applet load="2or1" size="450" color="white" frame="true" align="right" spinBox="true" caption="2or1, resolution 2.500&Aring;" /> '''RECOGNITION OF A DN...)
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[[Image:2or1.jpg|left|200px]]<br /><applet load="2or1" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2or1, resolution 2.500&Aring;" />
 
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'''RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION'''<br />
 
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==Overview==
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==RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION==
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The repressors of temperate bacteriophages such as 434 and lambda control, transcription by binding to a set of DNA operator sites. The different, affinity of repressor for each of these sites ensures efficient, regulation. High-resolution x-ray crystallography was used to study the, DNA-binding domain of phage 434 repressor in complex with a synthetic DNA, operator. The structure shows recognition of the operator by direct, interactions with base pairs in the major groove, combined with the, sequence-dependent ability of DNA to adopt the required conformation on, binding repressor. In particular, a network of three-centered bifurcated, hydrogen bonds among base pairs in the operator helps explain why 434, repressor prefers certain sites over others. These bonds, which stabilize, the conformation of the bound DNA, can form only with certain sequences.
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<StructureSection load='2or1' size='340' side='right'caption='[[2or1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2or1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Phage_434 Phage 434]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OR1 FirstGlance]. <br>
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2OR1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_434 Bacteriophage 434]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OR1 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2or1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2or1 OCA], [https://pdbe.org/2or1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2or1 RCSB], [https://www.ebi.ac.uk/pdbsum/2or1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2or1 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Recognition of a DNA operator by the repressor of phage 434: a view at high resolution., Aggarwal AK, Rodgers DW, Drottar M, Ptashne M, Harrison SC, Science. 1988 Nov 11;242(4880):899-907. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3187531 3187531]
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== Function ==
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[[Category: Bacteriophage 434]]
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[https://www.uniprot.org/uniprot/RPC1_BP434 RPC1_BP434] Binds to two sets of three contiguous operator sites in the phage genome.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Aggarwal, A.K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Drottar, M.]]
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Check<jmol>
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[[Category: Harrison, S.C.]]
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<jmolCheckbox>
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[[Category: Ptashne, M.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/2or1_consurf.spt"</scriptWhenChecked>
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[[Category: Rodgers, D.W.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: double helix]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: protein-dna complex]]
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2or1 ConSurf].
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:15:49 2007''
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Phage 434]]
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[[Category: Aggarwal AK]]
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[[Category: Drottar M]]
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[[Category: Harrison SC]]
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[[Category: Ptashne M]]
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[[Category: Rodgers DW]]

Current revision

RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION

PDB ID 2or1

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