2owh

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(New page: 200px<br /><applet load="2owh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2owh, resolution 2.50&Aring;" /> '''Structure of an earl...)
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[[Image:2owh.jpg|left|200px]]<br /><applet load="2owh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2owh, resolution 2.50&Aring;" />
 
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'''Structure of an early-microsecond photolyzed state of CO-bjFixLH'''<br />
 
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==Overview==
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==Structure of an early-microsecond photolyzed state of CO-bjFixLH==
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The FixL protein of Bradyrhizobium japonicum is a dimeric oxygen sensor, responsible for initiating regulation of transcription of genes encoding, proteins involved in nitrogen fixation and oxidative stress. It consists, of an N-terminal heme-bound PAS domain, denoted bjFixLH, and a C-terminal, histidine kinase domain whose enzymatic activity depends on the ligation, state of the heme. To investigate the molecular basis for this dependence, and the dynamics associated with conversion between ligated and unligated, states, we have conducted time-resolved Laue diffraction studies of CO, recombination in bjFixLH. Time-dependent difference Fourier maps from 1, micros to 10 ms after photolysis of the heme-CO bond show movement of the, side chain of Leu236 and the H and I beta-strands into the ligand binding, pocket formerly occupied by CO. Long-range conformational changes are, evident in the protein, driven by relaxation of steric interactions, between the bound ligand and amino acid side chains and/or changes in heme, stereochemistry. These structural changes fully reverse as CO rebinds to, the heme. Spectroscopic measurements of CO recombination kinetics in, bjFixLH crystals relate the behavior of crystalline bjFixLH to solution, and provide a framework for our time-resolved crystallographic, experiments. Analysis of the time-dependent difference Fourier maps by, singular value decomposition reveals that only one significant singular, value accounts for the data. Thus only two structural states are present, the photolyzed and the CO-bound states. The first left singular vector, represents the difference in density between these two states and shows, features common to difference maps calculated from the static CO and deoxy, states. The first right singular vector represents the time course of this, difference density and agrees well with the CO recombination kinetics, measured spectroscopically. We refine the structure of the photolyzed, state present in the early-microsecond time range and find that it does, not differ significantly in conformation from static, deoxy bjFixLH. Thus, structural relaxation from CO-bound to deoxy bjFixLH is complete in less, than 1 micros.
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<StructureSection load='2owh' size='340' side='right'caption='[[2owh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2owh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OWH FirstGlance]. <br>
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2OWH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OWH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2owh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2owh OCA], [https://pdbe.org/2owh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2owh RCSB], [https://www.ebi.ac.uk/pdbsum/2owh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2owh ProSAT]</span></td></tr>
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Time-resolved crystallographic studies of the heme domain of the oxygen sensor FixL: structural dynamics of ligand rebinding and their relation to signal transduction., Key J, Srajer V, Pahl R, Moffat K, Biochemistry. 2007 Apr 24;46(16):4706-15. Epub 2007 Mar 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17385895 17385895]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FIXL_BRADU FIXL_BRADU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/2owh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2owh ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bradyrhizobium japonicum]]
[[Category: Bradyrhizobium japonicum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Key, J.]]
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[[Category: Key J]]
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[[Category: Moffat, K.]]
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[[Category: Moffat K]]
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[[Category: Pahl, R.]]
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[[Category: Pahl R]]
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[[Category: Srajer, V.]]
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[[Category: Srajer V]]
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[[Category: HEM]]
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[[Category: heme]]
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[[Category: laue diffraction]]
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[[Category: oxygen sensor]]
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[[Category: pas domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:20:22 2007''
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Current revision

Structure of an early-microsecond photolyzed state of CO-bjFixLH

PDB ID 2owh

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