2p1o

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(New page: 200px<br /><applet load="2p1o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2p1o, resolution 1.90&Aring;" /> '''Mechanism of Auxin P...)
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[[Image:2p1o.gif|left|200px]]<br /><applet load="2p1o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p1o, resolution 1.90&Aring;" />
 
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'''Mechanism of Auxin Perception by the TIR1 ubiquitin ligase'''<br />
 
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==Overview==
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==Mechanism of Auxin Perception by the TIR1 ubiquitin ligase==
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Auxin is a pivotal plant hormone that controls many aspects of plant, growth and development. Perceived by a small family of F-box proteins, including transport inhibitor response 1 (TIR1), auxin regulates gene, expression by promoting SCF ubiquitin-ligase-catalysed degradation of the, Aux/IAA transcription repressors, but how the TIR1 F-box protein senses, and becomes activated by auxin remains unclear. Here we present the, crystal structures of the Arabidopsis TIR1-ASK1 complex, free and in, complexes with three different auxin compounds and an Aux/IAA substrate, peptide. These structures show that the leucine-rich repeat domain of TIR1, contains an unexpected inositol hexakisphosphate co-factor and recognizes, auxin and the Aux/IAA polypeptide substrate through a single surface, pocket. Anchored to the base of the TIR1 pocket, auxin binds to a, partially promiscuous site, which can also accommodate various auxin, analogues. Docked on top of auxin, the Aux/IAA substrate peptide occupies, the rest of the TIR1 pocket and completely encloses the hormone-binding, site. By filling in a hydrophobic cavity at the protein interface, auxin, enhances the TIR1-substrate interactions by acting as a 'molecular glue'., Our results establish the first structural model of a plant hormone, receptor.
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<StructureSection load='2p1o' size='340' side='right'caption='[[2p1o]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2p1o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P1O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=NLA:NAPHTHALEN-1-YL-ACETIC+ACID'>NLA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p1o OCA], [https://pdbe.org/2p1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p1o RCSB], [https://www.ebi.ac.uk/pdbsum/2p1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p1o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SKP1A_ARATH SKP1A_ARATH] Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends of the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.<ref>PMID:10528262</ref> <ref>PMID:10398681</ref> <ref>PMID:10500191</ref> <ref>PMID:11526079</ref> <ref>PMID:12970487</ref> <ref>PMID:14688296</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/2p1o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p1o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Auxin is a pivotal plant hormone that controls many aspects of plant growth and development. Perceived by a small family of F-box proteins including transport inhibitor response 1 (TIR1), auxin regulates gene expression by promoting SCF ubiquitin-ligase-catalysed degradation of the Aux/IAA transcription repressors, but how the TIR1 F-box protein senses and becomes activated by auxin remains unclear. Here we present the crystal structures of the Arabidopsis TIR1-ASK1 complex, free and in complexes with three different auxin compounds and an Aux/IAA substrate peptide. These structures show that the leucine-rich repeat domain of TIR1 contains an unexpected inositol hexakisphosphate co-factor and recognizes auxin and the Aux/IAA polypeptide substrate through a single surface pocket. Anchored to the base of the TIR1 pocket, auxin binds to a partially promiscuous site, which can also accommodate various auxin analogues. Docked on top of auxin, the Aux/IAA substrate peptide occupies the rest of the TIR1 pocket and completely encloses the hormone-binding site. By filling in a hydrophobic cavity at the protein interface, auxin enhances the TIR1-substrate interactions by acting as a 'molecular glue'. Our results establish the first structural model of a plant hormone receptor.
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==About this Structure==
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Mechanism of auxin perception by the TIR1 ubiquitin ligase.,Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, Zheng N Nature. 2007 Apr 5;446(7136):640-5. PMID:17410169<ref>PMID:17410169</ref>
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2P1O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with IHP and NLA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2P1O OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Mechanism of auxin perception by the TIR1 ubiquitin ligase., Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, Zheng N, Nature. 2007 Apr 5;446(7136):640-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17410169 17410169]
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</div>
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[[Category: Arabidopsis thaliana]]
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<div class="pdbe-citations 2p1o" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Calderon-Villalobos, L.I.A.]]
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[[Category: Estelle, M.]]
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[[Category: Robinson, C.V.]]
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[[Category: Sharon, M.]]
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[[Category: Tan, X.]]
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[[Category: Zheng, N.]]
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[[Category: IHP]]
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[[Category: NLA]]
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[[Category: f-box]]
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[[Category: leucine rich repeat]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:22:55 2007''
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==See Also==
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*[[Transport inhibitor response 1|Transport inhibitor response 1]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Arabidopsis thaliana]]
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[[Category: Large Structures]]
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[[Category: Calderon-Villalobos LIA]]
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[[Category: Estelle M]]
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[[Category: Robinson CV]]
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[[Category: Sharon M]]
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[[Category: Tan X]]
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[[Category: Zheng N]]

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Mechanism of Auxin Perception by the TIR1 ubiquitin ligase

PDB ID 2p1o

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