2o33

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{{Seed}}
 
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[[Image:2o33.png|left|200px]]
 
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==Solution structure of U2 snRNA stem I from S. cerevisiae==
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The line below this paragraph, containing "STRUCTURE_2o33", creates the "Structure Box" on the page.
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<StructureSection load='2o33' size='340' side='right'caption='[[2o33]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2o33]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O33 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o33 OCA], [https://pdbe.org/2o33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o33 RCSB], [https://www.ebi.ac.uk/pdbsum/2o33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o33 ProSAT]</span></td></tr>
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{{STRUCTURE_2o33| PDB=2o33 | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The spliceosome is a dynamic ribonucleoprotein complex responsible for the removal of intron sequences from pre-messenger RNA. The highly conserved 5' end of the U2 small nuclear RNA (snRNA) makes key base-pairing interactions with the intron branch point sequence and U6 snRNA. U2 stem I, a stem-loop located in the 5' region of U2, has been implicated in spliceosome assembly and may modulate the folding of the U2 and U6 snRNAs in the spliceosome active site. Here we present the NMR structures of U2 stem I from human and Saccharomyces cerevisiae. These sequences represent the two major classes of U2 stem I, distinguished by the identity of tandem wobble pairs (UU/UU in yeast and CA/GU in human) and the presence of post-transcriptional modifications (four 2'-O-methyl groups and two pseudouracils in human). The structures reveal that the UU/UU and CA/GU tandem wobble pairs are nearly isosteric. The tandem wobble pairs separate two thermodynamically distinct regions of Watson-Crick base pairs, with the modified nucleotides in human stem I conferring a significant increase in stability. We hypothesize that the separate thermodynamic stabilities of U2 stem I exist to allow the structure to transition through different folded conformations during spliceosome assembly and catalysis.
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===Solution structure of U2 snRNA stem I from S. cerevisiae===
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Structure and thermodynamics of a conserved U2 snRNA domain from yeast and human.,Sashital DG, Venditti V, Angers CG, Cornilescu G, Butcher SE RNA. 2007 Mar;13(3):328-38. Epub 2007 Jan 22. PMID:17242306<ref>PMID:17242306</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17242306}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2o33" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17242306 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17242306}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2O33 is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O33 OCA].
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[[Category: Angers CA]]
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[[Category: Butcher SE]]
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==Reference==
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[[Category: Cornilescu G]]
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<ref group="xtra">PMID:17242306</ref><references group="xtra"/>
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[[Category: Sashital DG]]
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[[Category: Angers, C A.]]
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[[Category: Venditti V]]
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[[Category: Butcher, S E.]]
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[[Category: Cornilescu, G.]]
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[[Category: Sashital, D G.]]
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[[Category: Venditti, V.]]
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[[Category: Ribonucleic acid]]
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[[Category: Rna]]
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[[Category: U2 snrna]]
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[[Category: Wobble base-pair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 19 08:45:06 2009''
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Current revision

Solution structure of U2 snRNA stem I from S. cerevisiae

PDB ID 2o33

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