2prd

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(New page: 200px<br /><applet load="2prd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2prd, resolution 2.0&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:2prd.jpg|left|200px]]<br /><applet load="2prd" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2prd, resolution 2.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF INORGANIC PYROPHOSPHATASE FROM THERMUS THERMOPHILUS'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF INORGANIC PYROPHOSPHATASE FROM THERMUS THERMOPHILUS==
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The 3-dimensional structure of inorganic pyrophosphatase from Thermus, thermophilus (T-PPase) has been determined by X-ray diffraction at 2.0 A, resolution and refined to R = 15.3%. The structure consists of an, antiparallel closed beta-sheet and 2 alpha-helices and resembles that of, the yeast enzyme in spite of the large difference in size (174 and 286, residues, respectively), little sequence similarity beyond the active, center (about 20%), and different oligomeric organization (hexameric and, dimeric, respectively). The similarity of the polypeptide folding in the 2, PPases provides a very strong argument in favor of an evolutionary, relationship between the yeast and bacterial enzymes. The same Greek-key, topology of the 5-stranded beta-barrel was found in the OB-fold proteins, the bacteriophage gene-5 DNA-binding protein, toxic-shock syndrome, toxin-1, and the major cold-shock protein of Bacillus subtilis. Moreover, all known nucleotide-binding sites in these proteins are located on the, same side of the beta-barrel as the active center in T-PPase. Analysis of, the active center of T-PPase revealed 17 residues of potential functional, importance, 16 of which are strictly conserved in all sequences of soluble, PPases. Their possible role in the catalytic mechanism is discussed on the, basis of the present crystal structure and with respect to site-directed, mutagenesis studies on the Escherichia coli enzyme. The observed, oligomeric organization of T-PPase allows us to suggest a possible, mechanism for the allosteric regulation of hexameric PPases.
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<StructureSection load='2prd' size='340' side='right'caption='[[2prd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2prd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PRD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2prd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2prd OCA], [https://pdbe.org/2prd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2prd RCSB], [https://www.ebi.ac.uk/pdbsum/2prd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2prd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IPYR_THET8 IPYR_THET8]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/2prd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2prd ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2PRD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2PRD OCA].
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*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of inorganic pyrophosphatase from Thermus thermophilus., Teplyakov A, Obmolova G, Wilson KS, Ishii K, Kaji H, Samejima T, Kuranova I, Protein Sci. 1994 Jul;3(7):1098-107. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7920256 7920256]
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[[Category: Large Structures]]
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[[Category: Inorganic diphosphatase]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Single protein]]
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[[Category: Teplyakov A]]
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[[Category: Thermus thermophilus]]
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[[Category: Teplyakov, A.]]
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[[Category: SO4]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:37:51 2007''
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CRYSTAL STRUCTURE OF INORGANIC PYROPHOSPHATASE FROM THERMUS THERMOPHILUS

PDB ID 2prd

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