This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3elo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:52, 16 February 2022) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3elo.png|left|200px]]
 
-
<!--
+
==Crystal Structure of Human Pancreatic Prophospholipase A2==
-
The line below this paragraph, containing "STRUCTURE_3elo", creates the "Structure Box" on the page.
+
<StructureSection load='3elo' size='340' side='right'caption='[[3elo]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3elo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ELO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ELO FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
-->
+
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PLA2G1B ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-
{{STRUCTURE_3elo| PDB=3elo | SCENE= }}
+
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3elo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3elo OCA], [https://pdbe.org/3elo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3elo RCSB], [https://www.ebi.ac.uk/pdbsum/3elo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3elo ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[[https://www.uniprot.org/uniprot/PA21B_HUMAN PA21B_HUMAN]] PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.<ref>PMID:19297324</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/3elo_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3elo ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Pancreatic phospholipase A2 (phospholipase A2 group 1B, G1B) belongs to the superfamily of secreted phospholipase A2 (PLA2) enzymes. G1B has been proposed to be a potential target for diseases such as hypertension, obesity, and diabetes. Human pancreatic prophospholipase A2 (pro-hG1B) is activated by cleavage of the first seven-residue propeptide (phospholipase A2 propeptide, PROP). However, questions still remain on the mode of action for pro-hG1B. In this work, we expressed pro-hG1B in Pichia pastoris and determined the crystal structure at 1.55-A resolution. The x-ray structure demonstrates that pro-hG1B forms a trimer. In addition, PROP occupies the catalytic cavity and can be self-cleaved at 37 degrees C. A new membrane-bound surface and activation mechanism are proposed based on the trimeric model of pro-hG1B. We also propose a new autoproteolytic mechanism for pro-hG1B by the reaction triad Asp49-Arg0-Ser(-2) that is similar to the serine protease catalytic triad.
-
===Crystal Structure of Human Pancreatic Prophospholipase A2===
+
Structural insight into the activation mechanism of human pancreatic prophospholipase A2.,Xu W, Yi L, Feng Y, Chen L, Liu J J Biol Chem. 2009 Jun 12;284(24):16659-66. Epub 2009 Mar 18. PMID:19297324<ref>PMID:19297324</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3elo" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_19297324}}, adds the Publication Abstract to the page
+
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 19297324 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19297324}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Human]]
-
3ELO is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ELO OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Chen, L]]
-
==Reference==
+
[[Category: Feng, Y]]
-
<ref group="xtra">PMID:19297324</ref><references group="xtra"/>
+
[[Category: Liu, J]]
-
[[Category: Homo sapiens]]
+
[[Category: Xu, W]]
-
[[Category: Chen, L.]]
+
[[Category: Yi, L]]
-
[[Category: Feng, Y.]]
+
-
[[Category: Liu, J.]]
+
-
[[Category: Xu, W.]]
+
-
[[Category: Yi, L.]]
+
-
[[Category: Calcium]]
+
[[Category: Human pancreatic prophospholipase a2]]
[[Category: Human pancreatic prophospholipase a2]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Lipid degradation]]
[[Category: Lipid degradation]]
[[Category: Metal-binding]]
[[Category: Metal-binding]]
-
[[Category: Polymorphism]]
 
[[Category: Secreted]]
[[Category: Secreted]]
[[Category: Trimeric]]
[[Category: Trimeric]]
- 
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 22 10:41:50 2009''
 

Current revision

Crystal Structure of Human Pancreatic Prophospholipase A2

PDB ID 3elo

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3elo"
Personal tools