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2rsl

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REFINEMENT OF GAMMA DELTA RESOLVASE REVEALS A STRIKINGLY FLEXIBLE MOLECULE

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Retrieved from "http://52.214.119.220/wiki/index.php/2rsl"

Categories: Escherichia coli | Large Structures | Rice PA | Steitz TA

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-[[Image:2rsl.jpg|left|200px]]<br /><applet load="2rsl" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2rsl, resolution 2.3&Aring;" />
-'''REFINEMENT OF GAMMA DELTA RESOLVASE REVEALS A STRIKINGLY FLEXIBLE MOLECULE'''<br />
-==Overview==
+==REFINEMENT OF GAMMA DELTA RESOLVASE REVEALS A STRIKINGLY FLEXIBLE MOLECULE==
-BACKGROUND: gamma delta resolvase is a 20.5 kDa enzyme that catalyzes a, site-specific recombination in the second step of the transposition of the, gamma delta transposon and requires no cofactors other than Mg2+ for, activity. Dimers of resolvase bind cooperatively to DNA at three inverted, repeat sequences of differing geometry but catalyze recombination at only, one site. RESULTS: The structure of the catalytic domain of gamma delta, resolvase, which provides the protein-protein interactions in the synaptic, complex, has been refined to an R-factor of 20% at 2.3 A resolution. The, structures of the three independent monomers in the asymmetric unit are, similar but not identical. Differences occur in the positions of surface, loops and in the overall twist of the central beta-sheet of the molecule., The crystal also gives two independent structures for the dimeric form of, the molecule, which also show significant differences in the relative, orientations of their subunits. CONCLUSION: Resolvase is an unusually, flexible protein. This conformational adaptability may be necessary to, allow each of the 12 resolvase subunits in the synaptic complex to play a, different but specific role in wrapping DNA, binding sites of differing, geometry and catalyzing recombination.
+<StructureSection load='2rsl' size='340' side='right'caption='[[2rsl]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2rsl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rsl 1rsl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RSL FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rsl OCA], [https://pdbe.org/2rsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rsl RCSB], [https://www.ebi.ac.uk/pdbsum/2rsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rsl ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TNR1_ECOLI TNR1_ECOLI] This protein catalyzes the site-specific recombination of the transposon and also regulates its frequency of transposition.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rs/2rsl_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rsl ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-RSL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 1RSL. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2RSL OCA].
+*[[Resolvase 3D structures|Resolvase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Refinement of gamma delta resolvase reveals a strikingly flexible molecule., Rice PA, Steitz TA, Structure. 1994 May 15;2(5):371-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8081753 8081753]
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Rice, P.A.]]
+[[Category: Rice PA]]
-[[Category: Steitz, T.A.]]
+[[Category: Steitz TA]]
-[[Category: SO4]]
-[[Category: site-specific recombinase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:58:49 2007''

2rsl

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REFINEMENT OF GAMMA DELTA RESOLVASE REVEALS A STRIKINGLY FLEXIBLE MOLECULE

Structural highlights

Function

Evolutionary Conservation

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