2zpa

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{{Seed}}
 
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[[Image:2zpa.png|left|200px]]
 
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==Crystal Structure of tRNA(Met) Cytidine Acetyltransferase==
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The line below this paragraph, containing "STRUCTURE_2zpa", creates the "Structure Box" on the page.
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<StructureSection load='2zpa' size='340' side='right'caption='[[2zpa]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zpa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZPA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2zpa| PDB=2zpa | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zpa OCA], [https://pdbe.org/2zpa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zpa RCSB], [https://www.ebi.ac.uk/pdbsum/2zpa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zpa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TMCA_ECOLI TMCA_ECOLI] Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). It recognizes the wobble base of tRNA(Met), thus distinguishing between tRNA(Met) and the structurally similar tRNA(Ile2).[HAMAP-Rule:MF_01886]<ref>PMID:18668122</ref> <ref>PMID:19322199</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zp/2zpa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zpa ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Post-transcriptional RNA modifications in the anticodon of transfer RNAs frequently contribute to the high fidelity of protein synthesis. In eubacteria, two genome-encoded transfer RNA (tRNA) species bear the same CAU sequence as the anticodons, which are differentiated by modified cytidines at the wobble positions. The elongator tRNA(Met) accepts an acetyl moiety at the wobble base to form N(4)-acetylcytidine (ac(4)C): an inherent modification ensures precise decoding of the AUG codon by strengthening C-G base-pair interaction and concurrently preventing misreading of the near cognate AUA codon. We have determined the crystal structure of tRNA(Met) cytidine acetyltransferase (TmcA) from Escherichia coli complexed with two natural ligands, acetyl-CoA and ADP, at 2.35 A resolution. The structure unexpectedly reveals an idiosyncratic RNA helicase module fused with a GCN5-related N-acetyltransferase (GNAT) fold, which intimately cross-interact. Taken together with the biochemical evidence, we further unravelled the function of acetyl-CoA as an enzyme-activating switch, and propose that an RNA helicase motor driven by ATP hydrolysis is used to deliver the wobble base to the active centre of the GNAT domain.
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===Crystal Structure of tRNA(Met) Cytidine Acetyltransferase===
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RNA helicase module in an acetyltransferase that modifies a specific tRNA anticodon.,Chimnaronk S, Suzuki T, Manita T, Ikeuchi Y, Yao M, Suzuki T, Tanaka I EMBO J. 2009 May 6;28(9):1362-73. Epub 2009 Mar 26. PMID:19322199<ref>PMID:19322199</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19322199}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2zpa" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19322199 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19322199}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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2ZPA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZPA OCA].
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[[Category: Large Structures]]
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[[Category: Chimnaronk S]]
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==Reference==
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[[Category: Manita T]]
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<ref group="xtra">PMID:19322199</ref><references group="xtra"/>
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[[Category: Tanaka I]]
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[[Category: Escherichia coli k12]]
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[[Category: Yao M]]
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[[Category: Chimnaronk, S.]]
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[[Category: Manita, T.]]
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[[Category: Tanaka, I.]]
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[[Category: Yao, M.]]
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[[Category: Acetyltransferase]]
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[[Category: Gcn5 acetyltransferase]]
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[[Category: Rna helicase]]
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[[Category: Rna modification enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:33:37 2009''
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Current revision

Crystal Structure of tRNA(Met) Cytidine Acetyltransferase

PDB ID 2zpa

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