3esu

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{{Seed}}
 
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[[Image:3esu.png|left|200px]]
 
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==Crystal structure of anthrax-neutralizing single-chain antibody 14b7==
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The line below this paragraph, containing "STRUCTURE_3esu", creates the "Structure Box" on the page.
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<StructureSection load='3esu' size='340' side='right'caption='[[3esu]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3esu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ESU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3esu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3esu OCA], [https://pdbe.org/3esu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3esu RCSB], [https://www.ebi.ac.uk/pdbsum/3esu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3esu ProSAT]</span></td></tr>
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{{STRUCTURE_3esu| PDB=3esu | SCENE= }}
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/3esu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3esu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The virulence of Bacillus anthracis is critically dependent on the cytotoxic components of the anthrax toxin, lethal factor (LF) and edema factor (EF). LF and EF gain entry into host cells through interactions with the protective antigen (PA), which binds to host cellular receptors such as CMG2. Antibodies that neutralize PA have been shown to confer protection in animal models and are undergoing intense clinical development. A murine monoclonal antibody, 14B7, has been reported to interact with domain 4 of PA (PAD4) and block its binding to CMG2. More recently, the 14B7 antibody was used as the platform for the selection of very high affinity, single-chain antibodies that have tremendous potential as a combination anthrax prophylactic and treatment. Here, we report the high-resolution X-ray structures of three high-affinity, single-chain antibodies in the 14B7 family; 14B7 and two high-affinity variants 1H and M18. In addition, we present the first neutralizing antibody-PA structure, M18 in complex with PAD4 at 3.8 A resolution. These structures provide insights into the mechanism of neutralization, and the effect of various mutations on antibody affinity, and enable a comparison between the binding of the M18 antibody and CMG2 with PAD4.
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===Crystal structure of anthrax-neutralizing single-chain antibody 14b7===
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Crystal structure of the engineered neutralizing antibody M18 complexed to domain 4 of the anthrax protective antigen.,Leysath CE, Monzingo AF, Maynard JA, Barnett J, Georgiou G, Iverson BL, Robertus JD J Mol Biol. 2009 Apr 3;387(3):680-93. Epub 2009 Feb 10. PMID:19361425<ref>PMID:19361425</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3esu" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19361425}}, adds the Publication Abstract to the page
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19361425 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19361425}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3ESU is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ESU OCA].
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==Reference==
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<ref group="xtra">PMID:19361425</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Georgiou, G.]]
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[[Category: Georgiou G]]
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[[Category: Iverson, B L.]]
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[[Category: Iverson BL]]
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[[Category: Maynard, J A.]]
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[[Category: Maynard JA]]
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[[Category: Monzingo, A F.]]
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[[Category: Monzingo AF]]
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[[Category: Robertus, J D.]]
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[[Category: Robertus JD]]
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[[Category: Immune system]]
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[[Category: Immunoglobulin]]
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[[Category: Monoclonal antibody]]
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[[Category: Single-chain fv]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:37:18 2009''
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Current revision

Crystal structure of anthrax-neutralizing single-chain antibody 14b7

PDB ID 3esu

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OCA

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