2wa8

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{{Seed}}
 
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[[Image:2wa8.png|left|200px]]
 
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==Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS - The Phe peptide structure==
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The line below this paragraph, containing "STRUCTURE_2wa8", creates the "Structure Box" on the page.
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<StructureSection load='2wa8' size='340' side='right'caption='[[2wa8]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wa8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WA8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wa8 OCA], [https://pdbe.org/2wa8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wa8 RCSB], [https://www.ebi.ac.uk/pdbsum/2wa8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wa8 ProSAT]</span></td></tr>
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{{STRUCTURE_2wa8| PDB=2wa8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CLPS_ECOLI CLPS_ECOLI] Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/2wa8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wa8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Escherichia coli, the ClpAP protease, together with the adaptor protein ClpS, is responsible for the degradation of proteins bearing an amino-terminal destabilizing amino acid (N-degron). Here, we determined the three-dimensional structures of ClpS in complex with three peptides, each having a different destabilizing residue--Leu, Phe or Trp--at its N terminus. All peptides, regardless of the identity of their N-terminal residue, are bound in a surface pocket on ClpS in a stereo-specific manner. Several highly conserved residues in this binding pocket interact directly with the backbone of the N-degron peptide and hence are crucial for the binding of all N-degrons. By contrast, two hydrophobic residues define the volume of the binding pocket and influence the specificity of ClpS. Taken together, our data suggest that ClpS has been optimized for the binding and delivery of N-degrons containing an N-terminal Phe or Leu.
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===STRUCTURAL BASIS OF N-END RULE SUBSTRATE RECOGNITION IN ESCHERICHIA COLI BY THE CLPAP ADAPTOR PROTEIN CLPS - THE PHE PEPTIDE STRUCTURE===
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Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS.,Schuenemann VJ, Kralik SM, Albrecht R, Spall SK, Truscott KN, Dougan DA, Zeth K EMBO Rep. 2009 May;10(5):508-14. Epub 2009 Apr 17. PMID:19373253<ref>PMID:19373253</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wa8" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19373253}}, adds the Publication Abstract to the page
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*[[ATP-dependent Clp protease adaptor protein 3D structures|ATP-dependent Clp protease adaptor protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19373253 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19373253}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2WA8 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WA8 OCA].
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==Reference==
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<ref group="xtra">PMID:19373253</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Albrecht, R.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Dougan, D A.]]
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[[Category: Large Structures]]
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[[Category: Kralik, S M.]]
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[[Category: Albrecht R]]
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[[Category: Schuenemann, V J.]]
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[[Category: Dougan DA]]
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[[Category: Spall, S K.]]
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[[Category: Kralik SM]]
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[[Category: Truscott, K N.]]
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[[Category: Schuenemann VJ]]
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[[Category: Zeth, K.]]
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[[Category: Spall SK]]
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[[Category: Clpa]]
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[[Category: Truscott KN]]
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[[Category: Clpp]]
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[[Category: Zeth K]]
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[[Category: Clp]]
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[[Category: N-end rule]]
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[[Category: Peptide-binding protein]]
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[[Category: Phe peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:42:47 2009''
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Current revision

Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS - The Phe peptide structure

PDB ID 2wa8

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