3eff

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (01:46, 21 November 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3eff.png|left|200px]]
 
-
<!--
+
==The Crystal Structure of Full-Length KcsA in its Closed Conformation==
-
The line below this paragraph, containing "STRUCTURE_3eff", creates the "Structure Box" on the page.
+
<StructureSection load='3eff' size='340' side='right'caption='[[3eff]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3eff]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EFF FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eff OCA], [https://pdbe.org/3eff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eff RCSB], [https://www.ebi.ac.uk/pdbsum/3eff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eff ProSAT]</span></td></tr>
-
{{STRUCTURE_3eff| PDB=3eff | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/3eff_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eff ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
KcsA is a proton-activated, voltage-modulated K(+) channel that has served as the archetype pore domain in the Kv channel superfamily. Here, we have used synthetic antigen-binding fragments (Fabs) as crystallographic chaperones to determine the structure of full-length KcsA at 3.8 A, as well as that of its isolated C-terminal domain at 2.6 A. The structure of the full-length KcsA-Fab complex reveals a well-defined, 4-helix bundle that projects approximately 70 A toward the cytoplasm. This bundle promotes a approximately 15 degrees bending in the inner bundle gate, tightening its diameter and shifting the narrowest point 2 turns of helix below. Functional analysis of the full-length KcsA-Fab complex suggests that the C-terminal bundle remains whole during gating. We suggest that this structure likely represents the physiologically relevant closed conformation of KcsA.
-
===The Crystal Structure of Full-Length KcsA in its Closed Conformation===
+
Crystal structure of full-length KcsA in its closed conformation.,Uysal S, Vasquez V, Tereshko V, Esaki K, Fellouse FA, Sidhu SS, Koide S, Perozo E, Kossiakoff A Proc Natl Acad Sci U S A. 2009 Apr 3. PMID:19346472<ref>PMID:19346472</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3eff" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_19346472}}, adds the Publication Abstract to the page
+
*[[Antibody 3D structures|Antibody 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 19346472 is the PubMed ID number.
+
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
-
-->
+
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
-
{{ABSTRACT_PUBMED_19346472}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
3EFF is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFF OCA].
+
</StructureSection>
-
 
+
[[Category: Large Structures]]
-
==Reference==
+
-
<ref group="xtra">PMID:19346472</ref><references group="xtra"/>
+
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Streptomyces lividans]]
[[Category: Streptomyces lividans]]
-
[[Category: Esaki, K.]]
+
[[Category: Esaki K]]
-
[[Category: Fellouse,F A.]]
+
[[Category: Fellouse FA]]
-
[[Category: Koide, S.]]
+
[[Category: Koide S]]
-
[[Category: Kossiakoff, A.]]
+
[[Category: Kossiakoff A]]
-
[[Category: Perozo, E.]]
+
[[Category: Perozo E]]
-
[[Category: Sidhu, S S.]]
+
[[Category: Sidhu SS]]
-
[[Category: Tereshko, T.]]
+
[[Category: Tereshko T]]
-
[[Category: Uysal, S.]]
+
[[Category: Uysal S]]
-
[[Category: Vasquez, V.]]
+
[[Category: Vasquez V]]
-
[[Category: Bulge helix]]
+
-
[[Category: Cell membrane]]
+
-
[[Category: Full length kcsa]]
+
-
[[Category: Ion transport]]
+
-
[[Category: Ionic channel]]
+
-
[[Category: Membrane]]
+
-
[[Category: Membrane protein]]
+
-
[[Category: Transmembrane]]
+
-
[[Category: Transport]]
+
-
[[Category: Voltage-gated channel]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:57:59 2009''
+

Current revision

The Crystal Structure of Full-Length KcsA in its Closed Conformation

PDB ID 3eff

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3eff"
Personal tools