2tbd

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(New page: 200px<br /><applet load="2tbd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2tbd" /> '''SV40 T ANTIGEN DNA-BINDING DOMAIN, NMR, 30 S...)
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[[Image:2tbd.jpg|left|200px]]<br /><applet load="2tbd" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2tbd" />
 
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'''SV40 T ANTIGEN DNA-BINDING DOMAIN, NMR, 30 STRUCTURES'''<br />
 
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==Overview==
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==SV40 T ANTIGEN DNA-BINDING DOMAIN, NMR, 30 STRUCTURES==
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The structure of the domain from simian virus 40 (SV40) large T-antigen, that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has, been determined by nuclear magnetic resonance spectroscopy. The overall, fold, consisting of a central five-stranded antiparallel beta-sheet, flanked by two alpha-helices on one side and one alpha-helix and one, 3(10)-helix on the other, is a new one. Previous mutational analyses have, identified two elements, termed A (approximately 152-155) and B2, (203-207), as essential for origin-specific recognition. These elements, form two closely juxtaposed loops that define a continuous surface on the, protein. The addition of a duplex oligonucleotide containing the origin, recognition pentanucleotide GAGGC induces chemical shift changes and slows, amide proton exchange in resonances from this region, indicating that this, surface directly contacts the DNA.
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<StructureSection load='2tbd' size='340' side='right'caption='[[2tbd]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2tbd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2TBD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2tbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2tbd OCA], [https://pdbe.org/2tbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2tbd RCSB], [https://www.ebi.ac.uk/pdbsum/2tbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2tbd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q98ZP7_SV40 Q98ZP7_SV40]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tb/2tbd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2tbd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA.
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==About this Structure==
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Solution structure of the origin DNA-binding domain of SV40 T-antigen.,Luo X, Sanford DG, Bullock PA, Bachovchin WW Nat Struct Biol. 1996 Dec;3(12):1034-9. PMID:8946857<ref>PMID:8946857</ref>
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2TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2TBD OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the origin DNA-binding domain of SV40 T-antigen., Luo X, Sanford DG, Bullock PA, Bachovchin WW, Nat Struct Biol. 1996 Dec;3(12):1034-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8946857 8946857]
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</div>
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[[Category: Simian virus 40]]
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<div class="pdbe-citations 2tbd" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Bachovchin, W.W.]]
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[[Category: Bullock, P.A.]]
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[[Category: Luo, X.]]
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[[Category: Sanford, D.G.]]
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[[Category: acetylation]]
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[[Category: dna-binding protein]]
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[[Category: early protein]]
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[[Category: nuclear protein]]
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[[Category: origin-binding domain]]
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[[Category: replication]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 14:03:47 2007''
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==See Also==
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*[[Large T Antigen|Large T Antigen]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Macaca mulatta polyomavirus 1]]
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[[Category: Bachovchin WW]]
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[[Category: Bullock PA]]
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[[Category: Luo X]]
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[[Category: Sanford DG]]

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SV40 T ANTIGEN DNA-BINDING DOMAIN, NMR, 30 STRUCTURES

PDB ID 2tbd

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