1mp3

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(New page: 200px<br /><applet load="1mp3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mp3, resolution 2.20&Aring;" /> '''L89T VARIANT OF S. E...)
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[[Image:1mp3.jpg|left|200px]]<br /><applet load="1mp3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mp3, resolution 2.20&Aring;" />
 
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'''L89T VARIANT OF S. ENTERICA RmlA'''<br />
 
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==Overview==
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==L89T VARIANT OF S. ENTERICA RmlA==
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In vitro "glycorandomization" is a chemoenzymatic approach for generating, diverse libraries of glycosylated biomolecules based on natural product, scaffolds. This technology makes use of engineered variants of specific, enzymes affecting metabolite glycosylation, particularly, nucleotidylyltransferases and glycosyltransferases. To expand the, repertoire of UDP/dTDP sugars readily available for glycorandomization, we, now report a structure-based engineering approach to increase the, diversity of alpha-d-hexopyranosyl phosphates accepted by Salmonella, enterica LT2 alpha-d-glucopyranosyl phosphate thymidylyltransferase, (E(p)). This article highlights the design rationale, determined substrate, specificity, and structural elucidation of three "designed" mutations, illustrating both the success and unexpected outcomes from this type of, approach. In addition, a single amino acid substitution in the, substrate-binding pocket (L89T) was found to significantly increase the, set of alpha-d-hexopyranosyl phosphates accepted by E(p) to include, alpha-d-allo-, alpha-d-altro-, and alpha-d-talopyranosyl phosphate. In, aggregate, our results provide valuable blueprints for altering, nucleotidylyltransferase specificity by design, which is the first step, toward in vitro glycorandomization.
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<StructureSection load='1mp3' size='340' side='right'caption='[[1mp3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mp3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MP3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mp3 OCA], [https://pdbe.org/1mp3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mp3 RCSB], [https://www.ebi.ac.uk/pdbsum/1mp3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mp3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9F7G8_SALER Q9F7G8_SALER] Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.[RuleBase:RU003706]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mp3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mp3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MP3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] with TTP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glucose-1-phosphate_thymidylyltransferase Glucose-1-phosphate thymidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.24 2.7.7.24] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MP3 OCA].
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*[[Glucose-1-phosphate thymidylyltransferase 3D structures|Glucose-1-phosphate thymidylyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering., Barton WA, Biggins JB, Jiang J, Thorson JS, Nikolov DB, Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13397-402. Epub 2002 Oct 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12374866 12374866]
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[[Category: Large Structures]]
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[[Category: Glucose-1-phosphate thymidylyltransferase]]
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[[Category: Salmonella enterica]]
[[Category: Salmonella enterica]]
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[[Category: Single protein]]
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[[Category: Barton WA]]
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[[Category: Barton, W.A.]]
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[[Category: Biggins JB]]
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[[Category: Biggins, J.B.]]
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[[Category: Jiang J]]
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[[Category: Jiang, J.]]
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[[Category: Nikolov DB]]
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[[Category: Nikolov, D.B.]]
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[[Category: Thorson JT]]
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[[Category: Thorson, J.T.]]
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[[Category: TTP]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:46:18 2007''
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Current revision

L89T VARIANT OF S. ENTERICA RmlA

PDB ID 1mp3

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