We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2khh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (19:12, 29 May 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2khh.jpg|left|200px]]
 
-
<!--
+
==Structural requirements for the UBA domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation Tho complex component Hpr1 and nucleoporin FxFG repeats==
-
The line below this paragraph, containing "STRUCTURE_2khh", creates the "Structure Box" on the page.
+
<StructureSection load='2khh' size='340' side='right'caption='[[2khh]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2khh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KHH FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2khh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2khh OCA], [https://pdbe.org/2khh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2khh RCSB], [https://www.ebi.ac.uk/pdbsum/2khh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2khh ProSAT]</span></td></tr>
-
{{STRUCTURE_2khh| PDB=2khh | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/2khh_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2khh ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The ubiquitin-associated (UBA) domain of the principal Saccharomyces cerevisiae mRNA nuclear export factor, Mex67, can bind both nuclear pore protein (nucleoporin) FG repeats and Hpr1, a component of the TREX.THO complex that functions to link transcription and export. Using fluorescence resonance energy transfer-based assays, we show here that Hpr1 and the FG repeats interact with overlapping binding sites on the Mex67 UBA domain. We present the solution structure of the Mex67 UBA domain (UBA-Mex67) complexed with a FXFG nucleoporin peptide and define residues engaged in the interaction and those involved in the FXFG-induced conformational change. We show by NMR titration that the binding of Hpr1 produces analogous changes in chemical shifts in similar regions of the UBA domain. Together the data presented here indicate that both Hpr1 and FXFG nucleoporins may bind in a similar way to the UBA-Mex67 domain. However, whereas binding of Hpr1 allows UBA-Mex67 to interact with tetra-ubiquitin, the complex between UBA-Mex67 and FXFG is unable to bind mono- or tetra-ubiquitin, suggesting that both substrate binding and also the nature of the substrate may influence the affinity of the UBA-Mex67 domain for ubiquitin.
-
===Structural requirements for the UBA domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation Tho complex component Hpr1 and nucleoporin FxFG repeats===
+
Structural requirements for the ubiquitin-associated domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation THO complex component Hpr1 and nucleoporin FXFG repeats.,Hobeika M, Brockmann C, Gruessing F, Neuhaus D, Divita G, Stewart M, Dargemont C J Biol Chem. 2009 Jun 26;284(26):17575-83. Epub 2009 Apr 28. PMID:19401465<ref>PMID:19401465</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_19401465}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2khh" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 19401465 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19401465}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2KHH is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KHH OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:19401465</ref><references group="xtra"/>
+
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Brockmann, C.]]
+
[[Category: Brockmann C]]
-
[[Category: Dargemont, C.]]
+
[[Category: Dargemont C]]
-
[[Category: Divita, G.]]
+
[[Category: Divita G]]
-
[[Category: Gruessing, F.]]
+
[[Category: Gruessing F]]
-
[[Category: Hobeika, M.]]
+
[[Category: Hobeika M]]
-
[[Category: Neuhaus, D.]]
+
[[Category: Neuhaus D]]
-
[[Category: Stewart, M.]]
+
[[Category: Stewart M]]
-
[[Category: Cytoplasm]]
+
-
[[Category: Fxfg]]
+
-
[[Category: Leucine-rich repeat]]
+
-
[[Category: Mex67]]
+
-
[[Category: Mrna export]]
+
-
[[Category: Mrna transport]]
+
-
[[Category: Nucleus]]
+
-
[[Category: Transport]]
+
-
[[Category: Transport protein]]
+
-
[[Category: Uba]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 13 10:07:26 2009''
+

Current revision

Structural requirements for the UBA domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation Tho complex component Hpr1 and nucleoporin FxFG repeats

PDB ID 2khh

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2khh"
Personal tools