3eto

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{{Seed}}
 
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[[Image:3eto.png|left|200px]]
 
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==2 Angstrom Xray structure of the NOTCH1 Negative Regulatory Region (NRR)==
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The line below this paragraph, containing "STRUCTURE_3eto", creates the "Structure Box" on the page.
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<StructureSection load='3eto' size='340' side='right'caption='[[3eto]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3eto]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ETO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_3eto| PDB=3eto | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eto OCA], [https://pdbe.org/3eto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eto RCSB], [https://www.ebi.ac.uk/pdbsum/3eto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eto ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/NOTC1_HUMAN NOTC1_HUMAN] Defects in NOTCH1 are a cause of aortic valve disease 1 (AOVD1) [MIM:[https://omim.org/entry/109730 109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.<ref>PMID:16025100</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/NOTC1_HUMAN NOTC1_HUMAN] Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Proteolytic resistance of Notch prior to ligand binding depends on the structural integrity of a negative regulatory region (NRR) of the receptor that immediately precedes the transmembrane segment. The NRR includes the 3 Lin12/Notch repeats and the juxtamembrane heterodimerization domain, the region of Notch1 most frequently mutated in T-cell acute lymphoblastic leukemia lymphoma (T-ALL). Here, we report the x-ray structure of the Notch1 NRR in its autoinhibited conformation. A key feature of the Notch1 structure that maintains its closed conformation is a conserved hydrophobic plug that sterically occludes the metalloprotease cleavage site. Crystal packing interactions involving a highly conserved, exposed face on the third Lin12/Notch repeat suggest that this site may normally be engaged in intermolecular or intramolecular protein-protein interactions. The majority of known T-ALL-associated point mutations map to residues in the hydrophobic interior of the Notch1 NRR. A novel mutation (H1545P), which alters a residue at the crystal-packing interface, leads to ligand-independent increases in signaling in reporter gene assays despite only mild destabilization of the NRR, suggesting that it releases the autoinhibitory clamp on the heterodimerization domain imposed by the Lin12/Notch repeats. The Notch1 NRR structure should facilitate a search for antibodies or compounds that stabilize the autoinhibited conformation.
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===2 Angstrom Xray structure of the NOTCH1 Negative Regulatory Region (NRR)===
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Structure of the Notch1-negative regulatory region: implications for normal activation and pathogenic signaling in T-ALL.,Gordon WR, Roy M, Vardar-Ulu D, Garfinkel M, Mansour MR, Aster JC, Blacklow SC Blood. 2009 Apr 30;113(18):4381-90. Epub 2008 Dec 15. PMID:19075186<ref>PMID:19075186</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3eto" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19075186 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19075186}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3ETO is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETO OCA].
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==Reference==
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<ref group="xtra">PMID:19075186</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Blacklow, S C.]]
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[[Category: Large Structures]]
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[[Category: Gordon, W R.]]
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[[Category: Blacklow SC]]
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[[Category: Activator]]
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[[Category: Gordon WR]]
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[[Category: Alpha-beta sandwich]]
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[[Category: Ank repeat]]
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[[Category: Autoinhibition]]
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[[Category: Calcium-binding]]
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[[Category: Developmental protein]]
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[[Category: Differentiation]]
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[[Category: Egf-like domain]]
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[[Category: Glycoprotein]]
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[[Category: Hd domain]]
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[[Category: Leukemia]]
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[[Category: Lnr repeat]]
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[[Category: Membrane]]
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[[Category: Metal-binding]]
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[[Category: Notch signaling pathway]]
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[[Category: Nucleus]]
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[[Category: Oncogene]]
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[[Category: Phosphoprotein]]
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[[Category: Polymorphism]]
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[[Category: Receptor]]
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[[Category: Sea domain]]
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[[Category: Signaling protein]]
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[[Category: T-all]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 20 16:03:13 2009''
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Current revision

2 Angstrom Xray structure of the NOTCH1 Negative Regulatory Region (NRR)

PDB ID 3eto

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