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2c2v

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{{Seed}}
 
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[[Image:2c2v.png|left|200px]]
 
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==Crystal structure of the CHIP-UBC13-UEV1a complex==
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The line below this paragraph, containing "STRUCTURE_2c2v", creates the "Structure Box" on the page.
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<StructureSection load='2c2v' size='340' side='right'caption='[[2c2v]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2c2v]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C2V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c2v OCA], [https://pdbe.org/2c2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c2v RCSB], [https://www.ebi.ac.uk/pdbsum/2c2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c2v ProSAT]</span></td></tr>
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{{STRUCTURE_2c2v| PDB=2c2v | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBE2N_HUMAN UBE2N_HUMAN] The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).<ref>PMID:10089880</ref> <ref>PMID:14562038</ref> <ref>PMID:19269966</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c2/2c2v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c2v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CHIP is a dimeric U box E3 ubiquitin ligase that binds Hsp90 and/or Hsp70 via its TPR-domain, facilitating ubiquitylation of chaperone bound client proteins. We have determined the crystal structure of CHIP bound to an Hsp90 C-terminal decapeptide. The structure explains how CHIP associates with either chaperone type and reveals an unusual asymmetric homodimer in which the protomers adopt radically different conformations. Additionally, we identified CHIP as a functional partner of Ubc13-Uev1a in formation of Lys63-linked polyubiquitin chains, extending CHIP's roles into ubiquitin regulation as well as targeted destruction. The structure of Ubc13-Uev1a bound to the CHIP U box domain defines the basis for selective cooperation of CHIP with specific ubiquitin-conjugating enzymes. Remarkably, the asymmetric arrangement of the TPR domains in the CHIP dimer occludes one Ubc binding site, so that CHIP operates with half-of-sites activity, providing an elegant means for coupling a dimeric chaperone to a single ubiquitylation system.
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===CRYSTAL STRUCTURE OF THE CHIP-UBC13-UEV1A COMPLEX===
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Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex.,Zhang M, Windheim M, Roe SM, Peggie M, Cohen P, Prodromou C, Pearl LH Mol Cell. 2005 Nov 23;20(4):525-38. PMID:16307917<ref>PMID:16307917</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2c2v" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16307917}}, adds the Publication Abstract to the page
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*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16307917 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16307917}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2C2V is a 12 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C2V OCA]. Relevant biological numbers for this protein at [http://bionumbers.hms.harvard.edu/search.aspx?log=y&task=searchbytrmorg&trm=%22heat+shock%22&org=%25 B10NUMB3R5]
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==Reference==
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<ref group="xtra">PMID:16307917</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Ubiquitin--protein ligase]]
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[[Category: Pearl LH]]
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[[Category: Pearl, L H.]]
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[[Category: Roe SM]]
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[[Category: Roe, S M.]]
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[[Category: Zhang M]]
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[[Category: Zhang, M.]]
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[[Category: Chaperone]]
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[[Category: E3 ligase]]
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[[Category: Heat-shock protein]]
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[[Category: Heat-shock protein complex]]
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[[Category: Tpr]]
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[[Category: Ubiquitinylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon May 25 07:53:48 2009''
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Current revision

Crystal structure of the CHIP-UBC13-UEV1a complex

PDB ID 2c2v

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