1iir

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(New page: 200px<br /><applet load="1iir" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iir, resolution 1.8&Aring;" /> '''Crystal Structure of ...)
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[[Image:1iir.gif|left|200px]]<br /><applet load="1iir" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1iir, resolution 1.8&Aring;" />
 
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'''Crystal Structure of UDP-glucosyltransferase GtfB'''<br />
 
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==Overview==
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==Crystal Structure of UDP-glucosyltransferase GtfB==
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BACKGROUND: Members of the vancomycin group of glycopeptide antibiotics, have an oxidatively crosslinked heptapeptide scaffold decorated at the, hydroxyl groups of 4-OH-Phegly4 or beta-OH-Tyr6 with mono- (residue 6) or, disaccharides (residue 4). The disaccharide in vancomycin itself is, L-vancosamine-1,2-glucose, and in chloroeremomycin it is, L-4-epi-vancosamine-1,2-glucose. The sugars and their substituents play an, important role in efficacy, particularly against vancomycin-resistant, pathogenic enterococci. RESULTS: The glucosyltransferase, GtfB, that, transfers the glucose residue from UDP-glucose to the 4-OH-Phegly4 residue, of the vancomycin aglycone, initiating the glycosylation pathway in, chloroeremomycin maturation, has been crystallized, and its structure has, been determined by X-ray analysis at 1.8 A resolution. The enzyme has a, two-domain structure, with a deep interdomain cleft identified as the, likely site of UDP-glucose binding. A hydrophobic patch on the surface of, the N-terminal domain is proposed to be the binding site of the aglycone, substrate. Mutagenesis has revealed Asp332 as the best candidate for the, general base in the glucosyltransfer reaction. CONCLUSIONS: The structure, of GtfB places it in a growing group of glycosyltransferases, including, Escherichia coli MurG and a beta-glucosyltransferase from T4 phage, which, together form a subclass of the glycosyltransferase superfamily and give, insights into the recognition of the NDP-sugar and aglycone cosubstrates., A single major interdomain linker between the N- and C- terminal domains, suggests that reprogramming of sugar transfer or aglycone recognition in, the antibiotic glycosyltransferases, including the glycopeptide and also, the macrolide antibiotics, will be facilitated by this structural, information.
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<StructureSection load='1iir' size='340' side='right'caption='[[1iir]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1iir]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis Amycolatopsis orientalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IIR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iir OCA], [https://pdbe.org/1iir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iir RCSB], [https://www.ebi.ac.uk/pdbsum/1iir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iir ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GTFB_AMYOR GTFB_AMYOR] Glucosyltransferase that transfers glucose to the 4-OH-Phegly(4) residue of vancomycin aglycone (AGV) to produce devancoaminyl-vancomycin (DVV) in the biosynthesis of glycopeptide antibiotic chloroeremomycin, a member of the vancomycin group of antibiotics.<ref>PMID:11294642</ref> <ref>PMID:9115410</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/1iir_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iir ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1IIR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Amycolatopsis_orientalis Amycolatopsis orientalis] with SO4 and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IIR OCA].
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics., Mulichak AM, Losey HC, Walsh CT, Garavito RM, Structure. 2001 Jul 3;9(7):547-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11470430 11470430]
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__TOC__
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</StructureSection>
[[Category: Amycolatopsis orientalis]]
[[Category: Amycolatopsis orientalis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Garavito, R.M.]]
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[[Category: Garavito RM]]
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[[Category: Losey, H.C.]]
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[[Category: Losey HC]]
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[[Category: Mulichak, A.M.]]
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[[Category: Mulichak AM]]
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[[Category: Walsh, C.T.]]
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[[Category: Walsh CT]]
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[[Category: MG]]
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[[Category: SO4]]
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[[Category: glycosyltransferase]]
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[[Category: rossmann fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:05:08 2007''
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Current revision

Crystal Structure of UDP-glucosyltransferase GtfB

PDB ID 1iir

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