3ejf

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{{Seed}}
 
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[[Image:3ejf.png|left|200px]]
 
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==Crystal structure of IBV X-domain at pH 8.5==
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The line below this paragraph, containing "STRUCTURE_3ejf", creates the "Structure Box" on the page.
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<StructureSection load='3ejf' size='340' side='right'caption='[[3ejf]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ejf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Infectious_bronchitis_virus Infectious bronchitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EJF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ejf OCA], [https://pdbe.org/3ejf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ejf RCSB], [https://www.ebi.ac.uk/pdbsum/3ejf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ejf ProSAT]</span></td></tr>
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{{STRUCTURE_3ejf| PDB=3ejf | SCENE= }}
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</table>
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== Function ==
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===Crystal structure of IBV X-domain at pH 8.5===
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[https://www.uniprot.org/uniprot/R1AB_IBVB R1AB_IBVB] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:11413307</ref> The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).<ref>PMID:11413307</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity).<ref>PMID:11413307</ref> The peptide p16 might be involved in the EGF signaling pathway.<ref>PMID:11413307</ref> The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).<ref>PMID:11413307</ref> The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity).<ref>PMID:11413307</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity).<ref>PMID:11413307</ref> Nsp9 is a ssRNA-binding protein (By similarity).<ref>PMID:11413307</ref> NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).<ref>PMID:11413307</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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(as it appears on PubMed at http://www.pubmed.gov), where 19177346 is the PubMed ID number.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/3ejf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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{{ABSTRACT_PUBMED_19177346}}
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ejf ConSurf].
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3EJF is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EJF OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:19177346</ref><references group="xtra"/>
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__TOC__
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[[Category: Viruses]]
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</StructureSection>
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[[Category: Hansen, G.]]
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[[Category: Infectious bronchitis virus]]
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[[Category: Hilgenfeld, R.]]
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[[Category: Large Structures]]
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[[Category: Piotrowski, Y.]]
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[[Category: Hansen G]]
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[[Category: Adrp]]
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[[Category: Hilgenfeld R]]
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[[Category: Coronavirus]]
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[[Category: Piotrowski Y]]
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[[Category: Hydrolase]]
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[[Category: Ibv]]
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[[Category: Macro domain]]
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[[Category: Nsp3]]
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[[Category: Ribosomal frameshifting]]
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[[Category: Rna-binding]]
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[[Category: X-domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 27 14:47:17 2009''
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Current revision

Crystal structure of IBV X-domain at pH 8.5

PDB ID 3ejf

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