3hct

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{{Seed}}
 
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[[Image:3hct.jpg|left|200px]]
 
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==Crystal structure of TRAF6 in complex with Ubc13 in the P1 space group==
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The line below this paragraph, containing "STRUCTURE_3hct", creates the "Structure Box" on the page.
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<StructureSection load='3hct' size='340' side='right'caption='[[3hct]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hct]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HCT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3hct| PDB=3hct | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hct OCA], [https://pdbe.org/3hct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hct RCSB], [https://www.ebi.ac.uk/pdbsum/3hct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hct ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRAF6_HUMAN TRAF6_HUMAN] E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation.<ref>PMID:8837778</ref> <ref>PMID:11057907</ref> <ref>PMID:16378096</ref> <ref>PMID:17135271</ref> <ref>PMID:18093978</ref> <ref>PMID:18758450</ref> <ref>PMID:19675569</ref> <ref>PMID:19713527</ref> <ref>PMID:12140561</ref> <ref>PMID:19465916</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hc/3hct_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hct ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tumor necrosis factor (TNF) receptor-associated factor (TRAF)-6 mediates Lys63-linked polyubiquitination for NF-kappaB activation via its N-terminal RING and zinc finger domains. Here we report the crystal structures of TRAF6 and its complex with the ubiquitin-conjugating enzyme (E2) Ubc13. The RING and zinc fingers of TRAF6 assume a rigid, elongated structure. Interaction of TRAF6 with Ubc13 involves direct contacts of the RING and the preceding residues, and the first zinc finger has a structural role. Unexpectedly, this region of TRAF6 is dimeric both in the crystal and in solution, different from the trimeric C-terminal TRAF domain. Structure-based mutagenesis reveals that TRAF6 dimerization is crucial for polyubiquitin synthesis and autoubiquitination. Fluorescence resonance energy transfer analysis shows that TRAF6 dimerization induces higher-order oligomerization of full-length TRAF6. The mismatch of dimeric and trimeric symmetry may provide a mode of infinite oligomerization that facilitates ligand-dependent signal transduction of many immune receptors.
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===Crystal structure of TRAF6 in complex with Ubc13 in the P1 space group===
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E2 interaction and dimerization in the crystal structure of TRAF6.,Yin Q, Lin SC, Lamothe B, Lu M, Lo YC, Hura G, Zheng L, Rich RL, Campos AD, Myszka DG, Lenardo MJ, Darnay BG, Wu H Nat Struct Mol Biol. 2009 Jun;16(6):658-66. Epub 2009 May 24. PMID:19465916<ref>PMID:19465916</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hct" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3HCT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HCT OCA].
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*[[TNF receptor-associated factor 3D structures|TNF receptor-associated factor 3D structures]]
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*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Ubiquitin--protein ligase]]
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[[Category: Large Structures]]
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[[Category: Campos, A D.]]
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[[Category: Campos AD]]
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[[Category: Darnay, B G.]]
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[[Category: Darnay BG]]
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[[Category: Hura, G.]]
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[[Category: Hura G]]
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[[Category: Lamothe, B.]]
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[[Category: Lamothe B]]
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[[Category: Lenardo, M J.]]
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[[Category: Lenardo MJ]]
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[[Category: Lin, S C.]]
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[[Category: Lin S-C]]
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[[Category: Lo, Y C.]]
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[[Category: Lo Y-C]]
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[[Category: Lu, M.]]
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[[Category: Lu M]]
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[[Category: Myszka, D G.]]
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[[Category: Myszka DG]]
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[[Category: Rich, R L.]]
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[[Category: Rich RL]]
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[[Category: Wu, H.]]
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[[Category: Wu H]]
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[[Category: Yin, Q.]]
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[[Category: Yin Q]]
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[[Category: Zheng, L.]]
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[[Category: Zheng L]]
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[[Category: Atp-binding]]
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[[Category: Beta-beta-alpha]]
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[[Category: Coiled coil]]
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[[Category: Cross-brace]]
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[[Category: Cytoplasm]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Isopeptide bond]]
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[[Category: Ligase]]
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[[Category: Metal-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: Signaling protein/ligase complex]]
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[[Category: Ubl conjugation]]
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[[Category: Ubl conjugation pathway]]
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[[Category: Zinc]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 27 15:29:06 2009''
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Current revision

Crystal structure of TRAF6 in complex with Ubc13 in the P1 space group

PDB ID 3hct

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