3fdq

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[[Image:3fdq.jpg|left|200px]]
 
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==Recognition of AT-rich DNA binding sites by the MogR Repressor==
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The line below this paragraph, containing "STRUCTURE_3fdq", creates the "Structure Box" on the page.
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<StructureSection load='3fdq' size='340' side='right'caption='[[3fdq]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fdq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FDQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FDQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fdq OCA], [https://pdbe.org/3fdq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fdq RCSB], [https://www.ebi.ac.uk/pdbsum/3fdq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fdq ProSAT]</span></td></tr>
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{{STRUCTURE_3fdq| PDB=3fdq | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MOGR_LISMO MOGR_LISMO] Transcriptional repressor of flagellar motility genes, such as flaA, during extracellular growth at 37 degrees Celsius and during intracellular infection. Binds directly to the gene promoter region and probably prevents RNA polymerase binding. At low temperatures, MogR repression activity is modulated by the DegU response regulator in an unknown mechanism. Required for full virulence.<ref>PMID:15302931</ref> <ref>PMID:16617375</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/3fdq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fdq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The MogR transcriptional repressor of the intracellular pathogen Listeria monocytogenes recognizes AT-rich binding sites in promoters of flagellar genes to downregulate flagellar gene expression during infection. We describe here the 1.8 A resolution crystal structure of MogR bound to the recognition sequence 5' ATTTTTTAAAAAAAT 3' present within the flaA promoter region. Our structure shows that MogR binds as a dimer. Each half-site is recognized in the major groove by a helix-turn-helix motif and in the minor groove by a loop from the symmetry-related molecule, resulting in a "crossover" binding mode. This oversampling through minor groove interactions is important for specificity. The MogR binding site has structural features of A-tract DNA and is bent by approximately 52 degrees away from the dimer. The structure explains how MogR achieves binding specificity in the AT-rich genome of L. monocytogenes and explains the evolutionary conservation of A-tract sequence elements within promoter regions of MogR-regulated flagellar genes.
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===Recognition of AT-rich DNA binding sites by the MogR Repressor===
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Recognition of AT-rich DNA binding sites by the MogR repressor.,Shen A, Higgins DE, Panne D Structure. 2009 May 13;17(5):769-77. PMID:19446532<ref>PMID:19446532</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19446532}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3fdq" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19446532 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19446532}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3FDQ is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FDQ OCA].
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==Reference==
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<ref group="xtra">PMID:19446532</ref><references group="xtra"/>
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[[Category: Listeria monocytogenes]]
[[Category: Listeria monocytogenes]]
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[[Category: Higgins, D E.]]
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[[Category: Higgins DE]]
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[[Category: Panne, D.]]
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[[Category: Panne D]]
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[[Category: Shen, A.]]
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[[Category: Shen A]]
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[[Category: Cytoplasm]]
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[[Category: Dna binding protein/dna complex]]
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[[Category: Dna-binding]]
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[[Category: Helix-turn-helix]]
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[[Category: Minor groove binding]]
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[[Category: Protein-dna complex]]
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[[Category: Repressor]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Virulence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 4 07:26:57 2009''
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Current revision

Recognition of AT-rich DNA binding sites by the MogR Repressor

PDB ID 3fdq

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