2kca

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{{Seed}}
 
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[[Image:2kca.png|left|200px]]
 
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==GP16==
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The line below this paragraph, containing "STRUCTURE_2kca", creates the "Structure Box" on the page.
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<StructureSection load='2kca' size='340' side='right'caption='[[2kca]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2kca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_SPP1 Bacillus phage SPP1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KCA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kca OCA], [https://pdbe.org/2kca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kca RCSB], [https://www.ebi.ac.uk/pdbsum/2kca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kca ProSAT]</span></td></tr>
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{{STRUCTURE_2kca| PDB=2kca | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HCP16_BPSPP HCP16_BPSPP] Functions as a stopper that is part of the head-tail connector and that locks the viral DNA in the capsid. Following tail attachment to the entry receptor, seems to open by a diaphragm-like motion, allowing the genome to exit the capsid through the tail tube to the host cell. During assembly, functions as a docking platform which the preassembled tail tapered by the head-tail joining protein gp17 can bind to.<ref>PMID:25991862</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kc/2kca_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kca ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In many bacterial viruses and in certain animal viruses, the double-stranded DNA genome enters and exits the capsid through a portal gatekeeper. We report a pseudoatomic structure of a complete portal system. The bacteriophage SPP1 gatekeeper is composed of dodecamers of the portal protein gp6, the adaptor gp15, and the stopper gp16. The solution structures of gp15 and gp16 were determined by NMR. They were then docked together with the X-ray structure of gp6 into the electron density of the approximately 1-MDa SPP1 portal complex purified from DNA-filled capsids. The resulting structure reveals that gatekeeper assembly is accompanied by a large rearrangement of the gp15 structure and by folding of a flexible loop of gp16 to form an intersubunit parallel beta-sheet that closes the portal channel. This stopper system prevents release of packaged DNA. Disulfide cross-linking between beta-strands of the stopper blocks the key conformational changes that control genome ejection from the virus at the beginning of host infection.
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===GP16===
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Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating.,Lhuillier S, Gallopin M, Gilquin B, Brasiles S, Lancelot N, Letellier G, Gilles M, Dethan G, Orlova EV, Couprie J, Tavares P, Zinn-Justin S Proc Natl Acad Sci U S A. 2009 May 26;106(21):8507-12. Epub 2009 May 11. PMID:19433794<ref>PMID:19433794</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19433794}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2kca" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19433794 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19433794}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus phage SPP1]]
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2KCA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_spp1 Bacillus phage spp1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KCA OCA].
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[[Category: Large Structures]]
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[[Category: Gallopin M]]
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==Reference==
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[[Category: Gilquin B]]
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<ref group="xtra">PMID:19433794</ref><references group="xtra"/>
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[[Category: Zinn-Justin S]]
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[[Category: Bacillus phage spp1]]
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[[Category: Gallopin, M.]]
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[[Category: Gilquin, B.]]
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[[Category: Zinn-Justin, S.]]
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[[Category: Beta-barrel]]
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[[Category: Virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 22:25:19 2009''
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Current revision

GP16

PDB ID 2kca

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