1zr6

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:45, 23 October 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1zr6.png|left|200px]]
 
-
<!--
+
==The crystal structure of an Acremonium strictum glucooligosaccharide oxidase reveals a novel flavinylation==
-
The line below this paragraph, containing "STRUCTURE_1zr6", creates the "Structure Box" on the page.
+
<StructureSection load='1zr6' size='340' side='right'caption='[[1zr6]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1zr6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sarocladium_strictum Sarocladium strictum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZR6 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_1zr6| PDB=1zr6 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zr6 OCA], [https://pdbe.org/1zr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zr6 RCSB], [https://www.ebi.ac.uk/pdbsum/1zr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zr6 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GOOX_SARSR GOOX_SARSR] Catalyzes the selective oxidation of C1 hydroxyl moieties on mono- and disaccharides with concomitant reduction of molecular oxygen to hydrogen peroxide. This results in the formation of the corresponding lactones, which typically undergo spontaneous hydrolysis. Glucooligosaccharide oxidase is able to oxidize the monosaccharide D-glucose as well as the disaccharides maltose, cellobiose, and lactose. In addition, it shows high selectivity for cello- and maltooligosaccharides, indicating that glucooligosaccharide oxidase prefers oligosaccharides with a beta-D-glucosyl unit on the reducing end and additional sugar units linked by alpha- or beta-1,4 glucosidic bonds.<ref>PMID:16332885</ref> <ref>PMID:1764476</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zr/1zr6_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zr6 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Glucooligosaccharide oxidase from Acremonium strictum has been screened for potential applications in oligosaccharide acid production and alternative carbohydrate detection, because it catalyzes the oxidation of glucose, maltose, lactose, cellobiose and cello- and maltooligosaccharides. We report the crystal structures of the enzyme and of its complex with an inhibitor, 5-amino-5-deoxy- cellobiono-1,5-lactam at 1.55- and 1.98-A resolution, respectively. Unexpectedly, the protein structure demonstrates the first known double attachment flavinylation, 6-S-cysteinyl, 8alpha-N1-histidyl FAD. The FAD cofactor is cross-linked to the enzyme via the C(6) atom and the 8alpha-methyl group of the isoalloxazine ring with Cys(130) and His(70), respectively. This sugar oxidase possesses an open carbohydrate-binding groove, allowing the accommodation of higher oligosaccharides. The complex structure suggests that this enzyme may prefer a beta-d-glucosyl residue at the reducing end with the conserved Tyr(429) acting as a general base to abstract the OH(1) proton in concert with the H(1) hydride transfer to the flavin N(5). Finally, a detailed comparison illustrates the structural conservation as well as the divergence between this protein and its related flavoenzymes.
-
===The crystal structure of an Acremonium strictum glucooligosaccharide oxidase reveals a novel flavinylation===
+
Crystal structure of glucooligosaccharide oxidase from Acremonium strictum: a novel flavinylation of 6-S-cysteinyl, 8alpha-N1-histidyl FAD.,Huang CH, Lai WL, Lee MH, Chen CJ, Vasella A, Tsai YC, Liaw SH J Biol Chem. 2005 Nov 18;280(46):38831-8. Epub 2005 Sep 9. PMID:16154992<ref>PMID:16154992</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16154992}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1zr6" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16154992 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16154992}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
1ZR6 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Acremonium_strictum Acremonium strictum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZR6 OCA].
+
[[Category: Sarocladium strictum]]
-
 
+
[[Category: Huang C-H]]
-
==Reference==
+
[[Category: Lai W-L]]
-
<ref group="xtra">PMID:16154992</ref><references group="xtra"/>
+
[[Category: Lee M-H]]
-
[[Category: Acremonium strictum]]
+
[[Category: Liaw S-H]]
-
[[Category: Huang, C H.]]
+
[[Category: Tsai Y-C]]
-
[[Category: Lai, W L.]]
+
-
[[Category: Lee, M H.]]
+
-
[[Category: Liaw, S H.]]
+
-
[[Category: Tsai, Y C.]]
+
-
[[Category: Alpha + beta]]
+
-
[[Category: Flavoenzyme]]
+
-
[[Category: Oxidoreductase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 22:57:34 2009''
+

Current revision

The crystal structure of an Acremonium strictum glucooligosaccharide oxidase reveals a novel flavinylation

PDB ID 1zr6

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zr6"
Personal tools