This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1na0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1na0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1na0, resolution 1.60&Aring;" /> '''Design of Stable alp...)
Current revision (07:54, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1na0.jpg|left|200px]]<br /><applet load="1na0" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1na0, resolution 1.60&Aring;" />
 
-
'''Design of Stable alpha-Helical Arrays from an Idealized TPR Motif'''<br />
 
-
==Overview==
+
==Design of Stable alpha-Helical Arrays from an Idealized TPR Motif==
-
The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif, that occurs in over 300 different proteins. In the different proteins, three to sixteen or more TPR motifs occur in tandem arrays and function to, mediate protein-protein interactions. The binding specificity of each TPR, protein is different, although the underlying structural motif is the, same. Here we describe a statistical approach to the design of an, idealized TPR motif. We present the high-resolution X-ray crystal, structures (to 1.55 and 1.6 A) of designed TPR proteins and describe their, solution properties and stability. A detailed analysis of these structures, provides an understanding of the TPR motif, how it is repeated to give, helical arrays with different superhelical twists, and how a very stable, framework may be constructed for future functional designs.
+
<StructureSection load='1na0' size='340' side='right'caption='[[1na0]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1na0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NA0 FirstGlance]. <br>
-
1NA0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ACT, PB, CL, NA, MG and IPT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NA0 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IPT:ISOPROPYL-1-BETA-D-THIOGALACTOSIDE'>IPT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1na0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1na0 OCA], [https://pdbe.org/1na0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1na0 RCSB], [https://www.ebi.ac.uk/pdbsum/1na0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1na0 ProSAT]</span></td></tr>
-
Design of stable alpha-helical arrays from an idealized TPR motif., Main ER, Xiong Y, Cocco MJ, D'Andrea L, Regan L, Structure. 2003 May;11(5):497-508. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737816 12737816]
+
</table>
-
[[Category: ]]
+
== Evolutionary Conservation ==
-
[[Category: Protein complex]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: Andrea, L.D.]]
+
Check<jmol>
-
[[Category: Cocco, M.]]
+
<jmolCheckbox>
-
[[Category: Main, E.]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1na0_consurf.spt"</scriptWhenChecked>
-
[[Category: Regan, L.]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
[[Category: Xiong, Y.]]
+
<text>to colour the structure by Evolutionary Conservation</text>
-
[[Category: ACT]]
+
</jmolCheckbox>
-
[[Category: CL]]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1na0 ConSurf].
-
[[Category: IPT]]
+
<div style="clear:both"></div>
-
[[Category: MG]]
+
__TOC__
-
[[Category: NA]]
+
</StructureSection>
-
[[Category: PB]]
+
[[Category: Large Structures]]
-
[[Category: design]]
+
[[Category: Unidentified]]
-
[[Category: tpr]]
+
[[Category: Cocco M]]
-
 
+
[[Category: D'Andrea L]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:48:37 2007''
+
[[Category: Main E]]
 +
[[Category: Regan L]]
 +
[[Category: Xiong Y]]

Current revision

Design of Stable alpha-Helical Arrays from an Idealized TPR Motif

PDB ID 1na0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1na0"
Personal tools