1vm1

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(New page: 200px<br /><applet load="1vm1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vm1, resolution 2.02&Aring;" /> '''STRUCTURE OF SHV-1 B...)
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[[Image:1vm1.gif|left|200px]]<br /><applet load="1vm1" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1vm1, resolution 2.02&Aring;" />
 
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'''STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM'''<br />
 
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==Overview==
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==STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM==
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Two species resulting from the reaction of the SHV-1 class A, beta-lactamase with the sulfone inhibitor tazobactam have been trapped at, 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic, form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of, tazobactam, is bonded to Ser130. It is proposed that the electron density, map of the crystal is a composite picture of two complexes, each with only, a single bound species. It is estimated that the two complexes exist in, the crystal in approximately equal populations. Results are discussed in, relation to the mechanism-based inhibition of class A beta-lactamases by, the similar inhibitors sulbactam and clavulanic acid.
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<StructureSection load='1vm1' size='340' side='right'caption='[[1vm1]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1vm1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1g56 1g56]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VM1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKR:ACRYLIC+ACID'>AKR</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=TAZ:TAZOBACTAM'>TAZ</scene>, <scene name='pdbligand=TBE:TAZOBACTAM+INTERMEDIATE'>TBE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vm1 OCA], [https://pdbe.org/1vm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vm1 RCSB], [https://www.ebi.ac.uk/pdbsum/1vm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vm1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLA1_KLEPN BLA1_KLEPN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vm/1vm1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vm1 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1VM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with MA4, TBE, AKR and TAZ as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1G56. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VM1 OCA].
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Inhibition of the SHV-1 beta-lactamase by sulfones: crystallographic observation of two reaction intermediates with tazobactam., Kuzin AP, Nukaga M, Nukaga Y, Hujer A, Bonomo RA, Knox JR, Biochemistry. 2001 Feb 13;40(6):1861-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11327849 11327849]
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[[Category: Beta-lactamase]]
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[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bonomo, R.A.]]
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[[Category: Bonomo RA]]
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[[Category: Hujer, A.]]
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[[Category: Hujer A]]
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[[Category: Knox, J.R.]]
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[[Category: Knox JR]]
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[[Category: Kuzin, A.P.]]
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[[Category: Kuzin AP]]
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[[Category: Nukaga, M.]]
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[[Category: Nukaga M]]
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[[Category: Nukaga, Y.]]
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[[Category: Nukaga Y]]
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[[Category: AKR]]
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[[Category: MA4]]
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[[Category: TAZ]]
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[[Category: TBE]]
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[[Category: beta-lactam hydrolase]]
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[[Category: beta-lactamase]]
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[[Category: detergent binding]]
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[[Category: inhibitor design]]
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[[Category: penicillinase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:49:06 2007''
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STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM

PDB ID 1vm1

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