3gz1

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{{Seed}}
 
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[[Image:3gz1.jpg|left|200px]]
 
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==Crystal structure of IpgC in complex with the chaperone binding region of IpaB==
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The line below this paragraph, containing "STRUCTURE_3gz1", creates the "Structure Box" on the page.
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<StructureSection load='3gz1' size='340' side='right'caption='[[3gz1]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3gz1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GZ1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_3gz1| PDB=3gz1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [https://pdbe.org/3gz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [https://www.ebi.ac.uk/pdbsum/3gz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gz1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IPGC_SHIFL IPGC_SHIFL] Assists the correct folding of nascent IpaB. Once it is bound to IpaB, it binds to IpaC and impedes their premature association that would lead to their degradation in the absence of IpcG.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/3gz1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gz1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The delivery of virulence factors into host cells through type III secretion systems is essential for enterobacterial pathogenesis. Molecular chaperones bind specifically to virulence factors in the bacterial cytosol before secretion. Invasion plasmid gene C (IpgC) is a chaperone that binds 2 essential virulence factors of Shigella: invasion plasmid antigens (Ipa) B and C. Here, we report the crystal structure of IpgC alone and in complex with the chaperone binding domain (CBD) of IpaB. The chaperone captures the CBD in an extended conformation that is stabilized by conserved residues lining the cleft. Analysis of the cocrystal structure reveals a sequence motif that is functional in the IpaB translocator class from different bacteria as determined by isothermal titration calorimetry. Our results show how translocators are chaperoned and may allow the design of inhibitors of enterobacterial diseases.
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===Crystal structure of IpgC in complex with the chaperone binding region of IpaB===
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IpaB-IpgC interaction defines binding motif for type III secretion translocator.,Lunelli M, Lokareddy RK, Zychlinsky A, Kolbe M Proc Natl Acad Sci U S A. 2009 May 28. PMID:19478065<ref>PMID:19478065</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19478065}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3gz1" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19478065 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19478065}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3GZ1 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA].
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==Reference==
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<ref group="xtra">PMID:19478065</ref><references group="xtra"/>
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[[Category: Shigella flexneri]]
[[Category: Shigella flexneri]]
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[[Category: Kolbe, M.]]
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[[Category: Kolbe M]]
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[[Category: Lokareddy, R K.]]
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[[Category: Lokareddy RK]]
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[[Category: Lunelli, M.]]
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[[Category: Lunelli M]]
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[[Category: Zychlinsky, A.]]
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[[Category: Zychlinsky A]]
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[[Category: Chaperone]]
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[[Category: Chaperone binding region]]
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[[Category: Cytoplasm]]
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[[Category: Membrane]]
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[[Category: Plasmid]]
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[[Category: Secreted]]
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[[Category: Tetratricopeptide repeat]]
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[[Category: Tpr]]
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[[Category: Transmembrane]]
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[[Category: Virulence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 17 10:14:21 2009''
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Current revision

Crystal structure of IpgC in complex with the chaperone binding region of IpaB

PDB ID 3gz1

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