1j23

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(New page: 200px<br /><applet load="1j23" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j23, resolution 1.78&Aring;" /> '''Crystal structure of...)
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[[Image:1j23.jpg|left|200px]]<br /><applet load="1j23" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1j23, resolution 1.78&Aring;" />
 
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'''Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain'''<br />
 
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==Overview==
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==Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain==
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The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched, structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. Here, we report the, domain organization of an archaeal homolog (Hef) of this family and the, X-ray crystal structure of the middle domain, with the nuclease activity., The nuclease domain architecture exhibits remarkable similarity to those, of restriction endonucleases, including the correspondence of the, GDX(n)ERKX(3)D signature motif in Hef to the PDX(n)(E/D)XK motif in, restriction enzymes. This structural study also suggests that the, XPF/Rad1/Mus81/ERCC1 proteins form a dimer through each interface of the, nuclease domain and the helix-hairpin-helix domain. Simultaneous, disruptions of both interfaces result in their dissociation into separate, monomers, with strikingly reduced endonuclease activities.
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<StructureSection load='1j23' size='340' side='right'caption='[[1j23]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1j23]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J23 FirstGlance]. <br>
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1J23 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J23 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j23 OCA], [https://pdbe.org/1j23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j23 RCSB], [https://www.ebi.ac.uk/pdbsum/1j23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j23 ProSAT]</span></td></tr>
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==Reference==
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</table>
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X-ray and biochemical anatomy of an archaeal XPF/Rad1/Mus81 family nuclease: similarity between its endonuclease domain and restriction enzymes., Nishino T, Komori K, Ishino Y, Morikawa K, Structure. 2003 Apr;11(4):445-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12679022 12679022]
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== Function ==
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[[Category: Pyrococcus furiosus dsm 3638]]
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[https://www.uniprot.org/uniprot/Q8TZH8_PYRFU Q8TZH8_PYRFU]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Ishino, Y.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Komori, K.]]
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Check<jmol>
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[[Category: Morikawa, K.]]
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<jmolCheckbox>
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[[Category: Nishino, T.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j2/1j23_consurf.spt"</scriptWhenChecked>
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[[Category: structure-specific endonuclease]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:57:17 2007''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j23 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pyrococcus furiosus DSM 3638]]
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[[Category: Ishino Y]]
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[[Category: Komori K]]
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[[Category: Morikawa K]]
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[[Category: Nishino T]]

Current revision

Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain

PDB ID 1j23

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