1w7u

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{{Seed}}
 
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[[Image:1w7u.png|left|200px]]
 
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==Photoproduct of the Wild-Type Aequorea victoria Green Fluorescent Protein after structural annealing at 170K==
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The line below this paragraph, containing "STRUCTURE_1w7u", creates the "Structure Box" on the page.
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<StructureSection load='1w7u' size='340' side='right'caption='[[1w7u]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1w7u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W7U FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene></td></tr>
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{{STRUCTURE_1w7u| PDB=1w7u | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w7u OCA], [https://pdbe.org/1w7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w7u RCSB], [https://www.ebi.ac.uk/pdbsum/1w7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w7u ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w7/1w7u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w7u ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Green fluorescent protein (GFP) fluoresces efficiently under blue excitation despite major electrostatic rearrangements resulting from photoionization of the chromophore and neutralization of Glu-222. A competing phototransformation process, which ionizes the chromophore and decarboxylates Glu-222, mimics the electrostatic and structural changes in the fluorescence photocycle. Structural and spectroscopic analysis of the cryogenically stabilized photoproduct at 100 K and a structurally annealed intermediate of the phototransformed protein at 170 K reveals distinct structural relaxations involving protein, chromophore, solvent, and photogenerated CO2. Strong structural changes of the 100 K photoproduct after decarboxylation appear exclusively within 15 angstroms of the chromophore and include the electrostatically driven perturbations of Gln-69, Cys-70, and water molecules in an H-bonding network connecting the chromophore. X-ray crystallography to 1.85 angstroms resolution and static and picosecond time-resolved IR spectroscopy identify structural mechanisms common to phototransformation and to the fluorescence photocycle. In particular, the appearance of a 1697 cm(-1) (+) difference band in both photocycle and phototransformation intermediates is a spectroscopic signature for the structural perturbation of Gln-69. This is taken as evidence for an electrostatically driven dynamic response that is common to both photoreaction pathways. The interactions between the chromophore and the perturbed residues and solvent are decreased or removed in the T203H single and T203H/Q69L double mutants, resulting in a strong reduction of the fluorescence quantum yield. This suggests that the electrostatic response to the transient formation of a buried charge in the wild type is important for the bright fluorescence.
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===PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN AFTER STRUCTURAL ANNEALING AT 170K===
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Ultrafast and low barrier motions in the photoreactions of the green fluorescent protein.,van Thor JJ, Georgiev GY, Towrie M, Sage JT J Biol Chem. 2005 Sep 30;280(39):33652-9. Epub 2005 Jul 20. PMID:16033764<ref>PMID:16033764</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1w7u" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16033764}}, adds the Publication Abstract to the page
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16033764 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16033764}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1W7U is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W7U OCA].
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==Reference==
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<ref group="xtra">PMID:16033764</ref><references group="xtra"/>
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Georgiev, G Y.]]
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[[Category: Large Structures]]
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[[Category: Sage, J T.]]
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[[Category: Georgiev GY]]
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[[Category: Thor, J J.Van.]]
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[[Category: Sage JT]]
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[[Category: Towrie, M.]]
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[[Category: Towrie M]]
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[[Category: Beta-barrel]]
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[[Category: Van Thor JJ]]
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[[Category: Bioluminescence]]
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[[Category: Fluorescent protein]]
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[[Category: Luminescent protein]]
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[[Category: Photoproduct]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 24 20:25:38 2009''
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Current revision

Photoproduct of the Wild-Type Aequorea victoria Green Fluorescent Protein after structural annealing at 170K

PDB ID 1w7u

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