3byb

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{{Seed}}
 
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[[Image:3byb.png|left|200px]]
 
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==Crystal structure of Textilinin-1, a Kunitz-type serine protease inhibitor from the Australian Common Brown snake venom==
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The line below this paragraph, containing "STRUCTURE_3byb", creates the "Structure Box" on the page.
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<StructureSection load='3byb' size='340' side='right'caption='[[3byb]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3byb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudonaja_textilis_textilis Pseudonaja textilis textilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BYB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ETX:2-ETHOXYETHANOL'>ETX</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3byb| PDB=3byb | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3byb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3byb OCA], [https://pdbe.org/3byb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3byb RCSB], [https://www.ebi.ac.uk/pdbsum/3byb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3byb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VKT1_PSETT VKT1_PSETT] Strongly inhibits plasmin (Ki=0.44 nM) and trypsin (Ki=0.42 nM). Has little effect on plasma (Ki=1870 nM) and tissue (Ki=12900 nM) kallikreins. In vivo, reduces bleeding in a small animal model.<ref>PMID:10847427</ref> <ref>PMID:12406072</ref> <ref>PMID:16707925</ref> <ref>PMID:19236611</ref> <ref>PMID:21843588</ref> <ref>PMID:23335990</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/by/3byb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3byb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Textilinin-1 is a Kunitz-type serine protease inhibitor isolated from the venom of the Australian common brown snake, Pseudonaja textilis. This molecule binds to and blocks the activity of a range of serine proteases, including plasmin and trypsin. Textilinin-1's ability to inhibit plasmin, a protease involved in fibrinolysis, has raised the possibility that it could be used as an alternative to aprotinin (Trasylol) as a systemic antibleeding agent in surgery. Here, the crystal structure of free recombinant textilinin-1 has been determined to 1.63 A, with three molecules observed in the asymmetric unit. All of these have a similar overall fold to aprotinin, except that the canonical loop for one of the molecules is inverted such that the side chain of the P1' residue, Val18, is partially buried by intramolecular contacts to Pro15, Thr13, and Ile36. In aprotinin, the P1' residue is Ala16, whose side chain is too small to form similar contacts. The loop inversion in textilinin-1 is facilitated by changes in backbone dihedral angles for the P1 and P2' residues, such that they alternate between values in the beta-sheet and alpha-helical regions of the Ramachandran plot. In a comparison with the structures of all other known Kunitz-type serine protease inhibitors, no such conformational variability has been observed. The presence of the bulkier valine as the P1' residue in textilinin-1 appears to be a major contributor to reducing the binding affinity for plasmin as compared to aprotinin (3.5 nm versus 0.053 nm) and could also account for an observed narrower binding specificity.
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===Crystal structure of Textilinin-1, a Kunitz-type serine protease inhibitor from the Australian Common Brown snake venom===
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Crystal structure of textilinin-1, a Kunitz-type serine protease inhibitor from the venom of the Australian common brown snake (Pseudonaja textilis).,Millers EK, Trabi M, Masci PP, Lavin MF, de Jersey J, Guddat LW FEBS J. 2009 Jun;276(11):3163-75. Epub 2009 Apr 28. PMID:19490116<ref>PMID:19490116</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19490116}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3byb" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19490116 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19490116}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3BYB is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudonaja_textilis_textilis Pseudonaja textilis textilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BYB OCA].
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==Reference==
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<ref group="xtra">PMID:19490116</ref><references group="xtra"/>
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[[Category: Pseudonaja textilis textilis]]
[[Category: Pseudonaja textilis textilis]]
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[[Category: Guddat, L W.]]
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[[Category: Guddat LW]]
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[[Category: Millers, E K.]]
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[[Category: Millers EK]]
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[[Category: Beta hairpin]]
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[[Category: Bpti-like]]
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[[Category: Hydrolase inhibitor]]
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[[Category: Kunitz-type protease inhibitor]]
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[[Category: Plasmin]]
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[[Category: Trypsin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:30:52 2009''
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Current revision

Crystal structure of Textilinin-1, a Kunitz-type serine protease inhibitor from the Australian Common Brown snake venom

PDB ID 3byb

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