3fd9

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{{Seed}}
 
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[[Image:3fd9.jpg|left|200px]]
 
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==Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa==
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The line below this paragraph, containing "STRUCTURE_3fd9", creates the "Structure Box" on the page.
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<StructureSection load='3fd9' size='340' side='right'caption='[[3fd9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fd9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FD9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3fd9| PDB=3fd9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fd9 OCA], [https://pdbe.org/3fd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fd9 RCSB], [https://www.ebi.ac.uk/pdbsum/3fd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fd9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0H2Z989_PSEAB A0A0H2Z989_PSEAB]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/3fd9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fd9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The opportunistic pathogen P. aeruginosa utilizes a type III secretion system (T3SS) to support acute infections in predisposed individuals. In this bacterium, expression of all T3SS-related genes is dependent on the AraC-type transcriptional activator ExsA. Before host contact, the T3SS is inactive and ExsA is repressed by the antiactivator protein ExsD. The repression, thought to occur through direct interactions between the two proteins, is relieved upon opening of the type III secretion (T3S) channel when secretion chaperone ExsC sequesters ExsD. We have solved the crystal structure of Delta20ExsD, a protease-resistant fragment of ExsD that lacks only the 20 amino terminal residues of the wild-type protein at 2.6 A. Surprisingly the structure revealed similarities between ExsD and the DNA binding domain of transcriptional repressor KorB. A model of an ExsD-DNA complex constructed on the basis of this homology produced a realistic complex that is supported by the prevalence of conserved residues in the putative DNA binding site and the results of differential scanning fluorimetry studies. Our findings challenge the currently held model that ExsD solely acts through interactions with ExsA and raise new questions with respect to the underlying mechanism of ExsA regulation.
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===Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa===
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Structural evidence suggests that antiactivator ExsD from Pseudomonas aeruginosa is a DNA binding protein.,Bernhards RC, Jing X, Vogelaar NJ, Robinson H, Schubot FD Protein Sci. 2009 Mar;18(3):503-13. PMID:19235906<ref>PMID:19235906</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3fd9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19235906 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19235906}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3FD9 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD9 OCA].
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[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
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[[Category: Schubot FD]]
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==Reference==
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<ref group="xtra">PMID:19235906</ref><references group="xtra"/>
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[[Category: Bacteria]]
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[[Category: Schubot, F D.]]
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[[Category: Anti-activator]]
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[[Category: Pseudomona]]
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[[Category: Repressor]]
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[[Category: Transcription regulation]]
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[[Category: Type iii secretion]]
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[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 09:05:42 2009''
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Current revision

Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa

PDB ID 3fd9

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