1j84

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(New page: 200px<br /><applet load="1j84" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j84, resolution 2.02&Aring;" /> '''STRUCTURE OF FAM17 C...)
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[[Image:1j84.jpg|left|200px]]<br /><applet load="1j84" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1j84, resolution 2.02&Aring;" />
 
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'''STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS WITH BOUND CELLOTETRAOSE'''<br />
 
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==Overview==
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==STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS WITH BOUND CELLOTETRAOSE==
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The crystal structure of the Clostridium cellulovorans, carbohydrate-binding module (CBM) belonging to family 17 has been solved, to 1.7 A resolution by multiple anomalous dispersion methods. CBM17 binds, to non-crystalline cellulose and soluble beta-1,4-glucans, with a minimal, binding requirement of cellotriose and optimal affinity for cellohexaose., The crystal structure of CBM17 complexed with cellotetraose solved at 2.0, A resolution revealed that binding occurs in a cleft on the surface of the, molecule involving two tryptophan residues and several charged amino, acids. Thermodynamic binding studies and alanine scanning mutagenesis in, combination with the cellotetraose complex structure allowed the mapping, of the CBM17 binding cleft. In contrast to the binding groove, characteristic of family 4 CBMs, family 17 CBMs appear to have a very, shallow binding cleft that may be more accessible to cellulose chains in, non-crystalline cellulose than the deeper binding clefts of family 4 CBMs., The structural differences in these two modules may reflect, non-overlapping binding niches on cellulose surfaces.
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<StructureSection load='1j84' size='340' side='right'caption='[[1j84]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1j84]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_cellulovorans Clostridium cellulovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J84 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j84 OCA], [https://pdbe.org/1j84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j84 RCSB], [https://www.ebi.ac.uk/pdbsum/1j84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j84 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P94622_CLOCL P94622_CLOCL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/1j84_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j84 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1J84 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulovorans Clostridium cellulovorans] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J84 OCA].
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study., Notenboom V, Boraston AB, Chiu P, Freelove AC, Kilburn DG, Rose DR, J Mol Biol. 2001 Dec 7;314(4):797-806. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11733998 11733998]
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[[Category: Cellulase]]
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[[Category: Clostridium cellulovorans]]
[[Category: Clostridium cellulovorans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Boraston, A.B.]]
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[[Category: Boraston AB]]
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[[Category: Chiu, P.]]
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[[Category: Chiu P]]
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[[Category: Freelove, A.C.J.]]
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[[Category: Freelove ACJ]]
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[[Category: Kilburn, D.G.]]
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[[Category: Kilburn DG]]
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[[Category: Notenboom, V.]]
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[[Category: Notenboom V]]
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[[Category: Rose, D.R.]]
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[[Category: Rose DR]]
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[[Category: CA]]
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[[Category: carbohydrate binding module]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:15:20 2007''
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Current revision

STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS WITH BOUND CELLOTETRAOSE

PDB ID 1j84

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