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1ru3

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(New page: 200px<br /><applet load="1ru3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ru3, resolution 2.20&Aring;" /> '''Crystal Structure of...)
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[[Image:1ru3.jpg|left|200px]]<br /><applet load="1ru3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ru3, resolution 2.20&Aring;" />
 
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'''Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans'''<br />
 
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==Overview==
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==Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans==
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In anaerobic microorganisms employing the acetyl-CoA pathway, acetyl-CoA, synthase (ACS) and CO dehydrogenase (CODH) form a complex (ACS/CODH) that, catalyzes the synthesis of acetyl-CoA from CO, a methyl group, and CoA., Previously, a [4Fe-4S] cubane bridged to a copper-nickel binuclear site, (active site cluster A of the ACS component) was identified in the, ACS(Mt)/CODH(Mt) from Moorella thermoacetica whereas another study, revealed a nickel-nickel site in the open form of ACS(Mt), and a, zink-nickel site in the closed form. The ACS(Ch) of the hydrogenogenic, bacterium Carboxydothermus hydrogenoformans was found to exist as an, 82.2-kDa monomer as well as in a 1:1 molar complex with the 73.3-kDa, CODHIII(Ch). Homogeneous ACS(Ch) and ACS(Ch)/CODHIII(Ch) catalyzed the, exchange between [1-(14)C]acetyl-CoA and (12)CO with specific activities, of 2.4 or 5.9 micromol of CO per min per mg, respectively, at 70 degrees C, and pH 6.0. They also catalyzed the synthesis of acetyl-CoA from CO, methylcobalamin, corrinoid iron-sulfur protein, and CoA with specific, activities of 0.14 or 0.91 micromol of acetyl-CoA formed per min per mg, respectively, at 70 degrees C and pH 7.3. The functional cluster A of, ACS(Ch) contains a Ni-Ni-[4Fe-4S] site, in which the positions proximal, and distal to the cubane are occupied by Ni ions. This result is apparent, from a positive correlation of the Ni contents and negative correlations, of the Cu or Zn contents with the acetyl-CoA/CO exchange activities of, different preparations of monomeric ACS(Ch), a 2.2-A crystal structure of, the dithionite-reduced monomer in an open conformation, and x-ray, absorption spectroscopy.
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<StructureSection load='1ru3' size='340' side='right'caption='[[1ru3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [https://pdbe.org/1ru3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ru3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ru3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P83789_CARHY P83789_CARHY]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ru/1ru3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ru3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RU3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans] with NI, SF4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA].
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*[[Acetyl-CoA synthase|Acetyl-CoA synthase]]
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*[[Acetyl-CoA synthase 3D structures|Acetyl-CoA synthase 3D structures]]
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==Reference==
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*[[Iron–sulfur proteins|Iron–sulfur proteins]]
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A functional Ni-Ni-[4Fe-4S] cluster in the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans., Svetlitchnyi V, Dobbek H, Meyer-Klaucke W, Meins T, Thiele B, Romer P, Huber R, Meyer O, Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):446-51. Epub 2003 Dec 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14699043 14699043]
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__TOC__
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</StructureSection>
[[Category: Carboxydothermus hydrogenoformans]]
[[Category: Carboxydothermus hydrogenoformans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dobbek, H.]]
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[[Category: Dobbek H]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Meins, T.]]
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[[Category: Meins T]]
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[[Category: Meyer, O.]]
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[[Category: Meyer O]]
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[[Category: Meyer-Klaucke, W.]]
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[[Category: Meyer-Klaucke W]]
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[[Category: Rmer, P.]]
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[[Category: Rmer P]]
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[[Category: Svetlitchnyi, V.]]
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[[Category: Svetlitchnyi V]]
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[[Category: Thiele, B.]]
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[[Category: Thiele B]]
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[[Category: GOL]]
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[[Category: NI]]
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[[Category: SF4]]
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[[Category: cluster a]]
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[[Category: nickel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:16:12 2007''
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Current revision

Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans

PDB ID 1ru3

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