1f56

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(New page: 200px<br /><applet load="1f56" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f56, resolution 2.05&Aring;" /> '''SPINACH PLANTACYANIN...)
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[[Image:1f56.gif|left|200px]]<br /><applet load="1f56" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f56, resolution 2.05&Aring;" />
 
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'''SPINACH PLANTACYANIN'''<br />
 
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==Overview==
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==SPINACH PLANTACYANIN==
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The crystal structure of the basic blue protein (plantacyanin) from, spinach (SBP) has been solved to a resolution of 2.05 A by molecular, replacement using the homologous protein from cucumber (CBP) as a model., Although the sequence identity of 58% between both proteins is only, moderate, the three-dimensional structures turned out to be highly similar, and the buried residues, which form the hydrophobic core of the protein, are almost completely conserved. However, the redox potentials of both, proteins differ by 40 mV, and a comparison of the two structures leads to, a single lysine replacing a proline in the cucumber sequence, which causes, a shift of the peptide chain and thus a subtle distortion of the copper, ligand geometry in respect to CBP. The crystal contained three monomers of, SBP in the asymmetric unit which show considerable variations in outer, loop regions owing to crystal packing, but not in the regions presumed to, be essential for redox partner recognition and redox potential fine tuning, of the copper centers. Still, bond length variations at the copper site, are at the same scale between the monomers of SBP as they are in respect, to CBP, indicating that in the oxidized state the protein does not impose, a high conformational strain on the copper.
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<StructureSection load='1f56' size='340' side='right'caption='[[1f56]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1f56]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F56 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F56 FirstGlance]. <br>
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1F56 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with SO4 and CU1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F56 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f56 OCA], [https://pdbe.org/1f56 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f56 RCSB], [https://www.ebi.ac.uk/pdbsum/1f56 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f56 ProSAT]</span></td></tr>
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Crystal structure of plantacyanin, a basic blue cupredoxin from spinach., Einsle O, Mehrabian Z, Nalbandyan R, Messerschmidt A, J Biol Inorg Chem. 2000 Oct;5(5):666-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11085657 11085657]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/O82080_SPIOL O82080_SPIOL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/1f56_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f56 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
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[[Category: Einsle, O.]]
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[[Category: Einsle O]]
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[[Category: Mehrabian, Z.]]
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[[Category: Mehrabian Z]]
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[[Category: Messerschmidt, A.]]
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[[Category: Messerschmidt A]]
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[[Category: Nalbandyan, R.]]
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[[Category: Nalbandyan R]]
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[[Category: CU1]]
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[[Category: SO4]]
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[[Category: beta barrel]]
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[[Category: copper protein]]
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[[Category: cupredoxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:17:52 2007''
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Current revision

SPINACH PLANTACYANIN

PDB ID 1f56

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