3eyw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:37, 6 September 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3eyw.jpg|left|200px]]
 
-
<!--
+
==Crystal structure of the C-terminal domain of E. coli KefC in complex with KefF==
-
The line below this paragraph, containing "STRUCTURE_3eyw", creates the "Structure Box" on the page.
+
<StructureSection load='3eyw' size='340' side='right'caption='[[3eyw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3eyw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EYW FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_3eyw| PDB=3eyw | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eyw OCA], [https://pdbe.org/3eyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eyw RCSB], [https://www.ebi.ac.uk/pdbsum/3eyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eyw ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KEFC_ECOLI KEFC_ECOLI] Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport.[https://www.uniprot.org/uniprot/KEFF_ECOLI KEFF_ECOLI] Regulatory subunit of a potassium efflux system that confers protection against electrophiles. Required for full activity of KefC. Shows redox enzymatic activity, but this enzymatic activity is not required for activation of KefC. Can use a wide range of substrates, including electrophilic quinones, and its function could be to reduce the redox toxicity of electrophilic quinones in parallel with acting as triggers for the KefC efflux system.[HAMAP-Rule:MF_01414]<ref>PMID:11053405</ref> <ref>PMID:19523906</ref> <ref>PMID:21742892</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
KTN (RCK) domains are nucleotide-binding folds that form the cytoplasmic regulatory complexes of various K+ channels and transporters. The mechanisms these proteins use to control their transmembrane pore-forming counterparts remains unclear despite numerous electrophysiological and structural studies. KTN (RCK) domains consistently crystallize as dimers within the asymmetric unit, forming a pronounced hinge between two Rossmann folds. We have previously proposed that modification of the hinge angle plays an important role in activating the associated membrane-integrated components of the channel or transporter. Here we report the structure of the C-terminal, KTN-bearing domain of the E. coli KefC K+ efflux system in association with the ancillary subunit, KefF, which is known to stabilize the conductive state. The structure of the complex and functional analysis of KefC variants reveal that control of the conformational flexibility inherent in the KTN dimer hinge is modulated by KefF and essential for regulation of KefC ion flux.
-
===Crystal structure of the C-terminal domain of E. coli KefC in complex with KefF===
+
KTN (RCK) domains regulate K+ channels and transporters by controlling the dimer-hinge conformation.,Roosild TP, Castronovo S, Miller S, Li C, Rasmussen T, Bartlett W, Gunasekera B, Choe S, Booth IR Structure. 2009 Jun 10;17(6):893-903. PMID:19523906<ref>PMID:19523906</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_19523906}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3eyw" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 19523906 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19523906}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Escherichia coli K-12]]
-
3EYW is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EYW OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Roosild TP]]
-
==Reference==
+
-
<ref group="xtra">PMID:19523906</ref><references group="xtra"/>
+
-
[[Category: Escherichia coli k12]]
+
-
[[Category: Roosild, T P.]]
+
-
[[Category: Antiport]]
+
-
[[Category: Channel regulation]]
+
-
[[Category: Inner membrane]]
+
-
[[Category: Ion transport]]
+
-
[[Category: K+ channel]]
+
-
[[Category: K+ efflux]]
+
-
[[Category: K+ transport]]
+
-
[[Category: Kefc]]
+
-
[[Category: Ktn]]
+
-
[[Category: Membrane]]
+
-
[[Category: Potassium]]
+
-
[[Category: Potassium transport]]
+
-
[[Category: Rck]]
+
-
[[Category: Transmembrane]]
+
-
[[Category: Transport protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:08:36 2009''
+

Current revision

Crystal structure of the C-terminal domain of E. coli KefC in complex with KefF

PDB ID 3eyw

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3eyw"
Personal tools