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1f6y

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(New page: 200px<br /><applet load="1f6y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f6y, resolution 2.20&Aring;" /> '''MAD CRYSTAL STRUCTUR...)
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[[Image:1f6y.jpg|left|200px]]<br /><applet load="1f6y" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f6y, resolution 2.20&Aring;" />
 
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'''MAD CRYSTAL STRUCTURE ANALYSIS OF METHYLTETRAHYDROFOLATE: CORRINOID/IRON-SULFUR PROTEIN METHYLTRANSFERASE (METR)'''<br />
 
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==Overview==
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==MAD CRYSTAL STRUCTURE ANALYSIS OF METHYLTETRAHYDROFOLATE: CORRINOID/IRON-SULFUR PROTEIN METHYLTRANSFERASE (METR)==
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BACKGROUND: Methyltetrahydrofolate, corrinoid iron-sulfur protein, methyltransferase (MeTr), catalyzes a key step in the Wood-Ljungdahl, pathway of carbon dioxide fixation. It transfers the N5-methyl group from, methyltetrahydrofolate (CH3-H4folate) to a cob(I)amide center in another, protein, the corrinoid iron-sulfur protein. MeTr is a member of a family, of proteins that includes methionine synthase and methanogenic enzymes, that activate the methyl group of methyltetra-hydromethano(or, -sarcino)pterin. We report the first structure of a protein in this, family. RESULTS: We determined the crystal structure of MeTr from, Clostridium thermoaceticum at 2.2 A resolution using multiwavelength, anomalous diffraction methods. The overall architecture presents a new, functional class of the versatile triose phosphate isomerase (TIM) barrel, fold. The MeTr tertiary structure is surprisingly similar to the crystal, structures of dihydropteroate synthetases despite sharing less than 20%, sequence identity. This homology permitted the methyl-H4folate binding, site to be modeled. The model suggests extensive conservation of the, pterin ring binding residues in the polar active sites of the, methyltransferases and dihydropteroate synthetases. The most significant, structural difference between these enzymes is in a loop structure above, the active site. It is quite open in MeTr, where it can be modeled as the, cobalamin binding site. CONCLUSIONS: The MeTr structure consists of a TIM, barrel that embeds methyl-H4folate and cobamide. All related, methyltransferases are predicted to fold into a similar TIM barrel pattern, and have a similar pterin and cobamide binding site. The observed, structure is consistent with either a 'front' (N5) or 'back' (C8a) side, protonation of CH3-H4folate, a key step that enhances the electrophilic, character of the methyl group, activating it for nucleophilic attack by, Co(I).
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<StructureSection load='1f6y' size='340' side='right'caption='[[1f6y]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1f6y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica_ATCC_39073 Moorella thermoacetica ATCC 39073]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F6Y FirstGlance]. <br>
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1F6Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F6Y OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f6y OCA], [https://pdbe.org/1f6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f6y RCSB], [https://www.ebi.ac.uk/pdbsum/1f6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f6y ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase., Doukov T, Seravalli J, Stezowski JJ, Ragsdale SW, Structure. 2000 Aug 15;8(8):817-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10997901 10997901]
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== Function ==
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[[Category: Moorella thermoacetica]]
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[https://www.uniprot.org/uniprot/ACSE_MOOTH ACSE_MOOTH] Methyltransferase that mediates the transfer of a N5-methyl group of (6S)-methyltetrahydrofolate to the 5-methoxybenzimidazolylcobamide cofactor of a corrinoid/Fe-S protein in the anaerobic acetyl-CoA pathway (Wood-Ljungdahl pathway) of carbon monoxide and carbon dioxide fixation.<ref>PMID:17172470</ref> <ref>PMID:22419154</ref> <ref>PMID:7928975</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Doukov, T.I.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Ragsdale, S.W.]]
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Check<jmol>
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[[Category: Seravalli, J.]]
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<jmolCheckbox>
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[[Category: Stezowski, J.J.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/1f6y_consurf.spt"</scriptWhenChecked>
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[[Category: carbon dioxide fixation]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: cobalamin]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: homodimer]]
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</jmolCheckbox>
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[[Category: methyltatrahydrofolate]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f6y ConSurf].
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[[Category: methyltransferase]]
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<div style="clear:both"></div>
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[[Category: one-carbon metabolism]]
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== References ==
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[[Category: tim barrel]]
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<references/>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:23:45 2007''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Moorella thermoacetica ATCC 39073]]
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[[Category: Doukov TI]]
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[[Category: Ragsdale SW]]
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[[Category: Seravalli J]]
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[[Category: Stezowski JJ]]

Current revision

MAD CRYSTAL STRUCTURE ANALYSIS OF METHYLTETRAHYDROFOLATE: CORRINOID/IRON-SULFUR PROTEIN METHYLTRANSFERASE (METR)

PDB ID 1f6y

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